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Mix-n-Stain CF Dye Small Ligand Labeling Kits

Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.

Mix-n-Stain CF Dye Small Ligand Labeling Kits
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Catalog #

92350, 92363, 92362, 92361, 92360, 92359, 92358, 92357, 92356, 92355, 92354, 92353, 92352, 92351, - 92364View allHide

Size

1 labeling

Conjugation

CF405M, CF408, CF488A, CF500, CF540, CF555, CF568, CF594, CF633, CF640R, CF647, CF650, CF660C, CF680, CF680R

Conjugation
Size
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1 labeling
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Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.


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Product Description

Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.  The ligand to be labeled must contain an aliphatic amine group that is biologically inert.  The amino group will form a covalent linkage with the provided reactive CF dyes.  For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain™  small ligand labeling kit flyer and the product information sheet for this product.

Mix-n-Stain™ small ligand labeling kits for cell surface labeling are available in your choice of 10 CF™ dye colors for labeling ligands for cell surface targets, and three CF™ dye colors for ligands that bind intracellular targets. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.

The complete list of colors include: CF405M, CF488A, CF568, CF594, CF633, CF640R, CF647, CF660C, CF680, CF680R, CF408, CF500, CF540, and CF650.

DyeCatalog no.Spectrally similar dyesEx (nm)Em (nm)Staining
CF™405M92362Pacific Blue™, BD Horizon™ V450408452Surface
CF™488A92350FITC, Alexa Fluor® 488490515Surface
CF™56892351Rhodamine Red, Alexa Fluor® 568562583Surface
CF™59492352Texas Red®, Alexa Fluor® 594593614Surface
CF™63392353Cy®5, Alexa Fluor® 633630650Surface
CF™640R92354Cy®5, Alexa Fluor® 647642662Surface
CF™64792359Cy®5, Alexa Fluor® 647650665Surface
CF™660C92360Alexa Fluor® 660667685Surface
CF™68092361Cy®5.5, Alexa Fluor® 660, IRDye®680LT681698Surface
CF™680R92355Cy®5.5, Alexa Fluor® 660, IRDye®680LT680701Surface
CF™40892356Pacific Blue™, BD Horizon™ V450408450Intracellular
CF™50092357FITC, Alexa Fluor® 488500510Intracellular
CF™54092358Tetramethylrhodamine (TAMRA), TRITC, Alexa Fluor® 546, Cy®3540570Intracellular
CF™55592364Alexa Fluor® 555, Cy®3, TRITC555585Intracellular
CF™65092363Cy®5, Alexa Fluor® 647650670Intracellular

Mix-n-Stain and CF dyes are covered by US and international patents, and are trademarks of Biotium, Inc. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation; Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Invitrogen Corp; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Biosciences.

References

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Support & Faq

Mix-n-Stain™ Antibody Labeling Kits

CF dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF dyes, the CF Dye Selection Guide, and our CF Dye FAQs.

Category: Mix-n-Stain™ Antibody Labeling Kits

← FAQs

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

See the Mix-n-Stain Kit Selection Guide, below:

Antibody formulationKit and protocol selectionNotes/Examples
Purified IgG containing:

• Sodium azide

• Less than 20 mM Tris

• Less than 10% glycerol
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• More than 20 mM Tris

• More than 10% glycerol

• Glycine

• Less than 0.5 ug/uL IgG
1. Choose the kit size based on the amount of IgG you wish to label

2. Perform ultrafiltration using the spin vial provided in the kit

3. Use the standard labeling protocol
If the amount of antibody you wish to label falls between two kits sizes, we recommend using the smaller kit size. For example, if you wish to label 20 ug IgG, choose the 5-20 ug-sized kit.
Purified IgG containing:

• Less than 4:1 BSA:IgG by weight

• Less than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the amount of IgG you wish to label

2. Use the standard labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS contain­ing 0.1% BSA. The BSA:IgG ratio by weight is 5 ug BSA:5 ug IgG or 1:1. Select a 5-20 ug-sized kit and follow the standard protocol.
Purified IgG containing:

• More than 4:1 BSA:IgG by weight

• More than 4:1 gelatin:IgG by weight
1. Choose the kit size based on the total amount of protein (IgG + BSA/gelatin) in the volume of antibody solution you wish to label

2. Use the modified labeling protocol
For example, if you wish to label 5 ug IgG in 5 uL PBS containing 1% BSA. The BSA:IgG ratio by weight is 50 ug BSA: 5 ug IgG or 10/1. Select a 50-100 ug-sized kit based on 55 ug of total protein in the labeling reaction and follow the modified labeling protocol. If the total protein amount falls between two kit sizes, you may get better results with the larger kit size.
IgG in ascites fluid1. Determine the concentration of protein in the ascites fluid

2. Choose the kit size based on the total amount of protein in the volume of ascites fluid you wish to label

3. Use the modified labeling protocol
For example, if you wish to label 10 uL ascites fluid containing 70 ug total protein. Select a 50-100 ug-sized kit based on 70 ug of total protein, and follow the modified labeling protocol.
Less than 5 ug purified IgG1. Add stabilizer protein such as BSA to the antibody to bring the total amount of protein 5 ug

2. Select a 5-20 ug kit and use the standard labeling protocol
For example, if you wish to label 1 ug IgG sample. Select a 5-20 ug-sized kit. Before labeling, add 4 ug BSA to 1 ug IgG and follow the standard labeling protocol.
IgG in:

• Serum

• Hybridoma cell culture supernatant
Not compatible due to the low concentration of IgG compared to other proteins in these formats; purify IgG before labelingUse protein A/G or a commercially available IgG clean-up kit to purify IgG. Determine the concentration of IgG, then select the appropriate kit/protocol based on the amount of IgG you wish to label and the buffer formulation after purification.

Category: Mix-n-Stain™ Antibody Labeling Kits

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CF™ Dyes

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

CF initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF dyes based on cyanine dye structures. Since then, our CF dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF™ Dyes

← FAQs

The exact chemical structures of CF dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF dye invention that makes CF dyes superior to other commercial dyes.

Category: CF™ Dyes

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.