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Mix-n-Stain CF Dye Small Ligand Labeling Kits

Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.

Mix-n-Stain CF Dye Small Ligand Labeling Kits
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Catalog #

92350, 92363, 92362, 92361, 92360, 92359, 92358, 92357, 92356, 92355, 92354, 92353, 92352, 92351, - 92364View allHide

Size

1 labeling

Conjugation

CF405M, CF408, CF488A, CF500, CF540, CF555, CF568, CF594, CF633, CF640R, CF647, CF650, CF660C, CF680, CF680R

Conjugation
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Shipping & Tax (CA only) not included in price. Learn more

Taxes: Tax is assessed in the state of California and is calculated during checkout based on your shipping information. Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout.


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Product Description

Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.  The ligand to be labeled must contain an aliphatic amine group that is biologically inert.  The amino group will form a covalent linkage with the provided reactive CF dyes.  For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain™  small ligand labeling kit flyer and the product information sheet for this product.

Mix-n-Stain™ small ligand labeling kits for cell surface labeling are available in your choice of 10 CF™ dye colors for labeling ligands for cell surface targets, and three CF™ dye colors for ligands that bind intracellular targets. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.

The complete list of colors include: CF405M, CF488A, CF568, CF594, CF633, CF640R, CF647, CF660C, CF680, CF680R, CF408, CF500, CF540, and CF650.

DyeCatalog no.Spectrally similar dyesEx (nm)Em (nm)Staining
CF™405M92362Pacific Blue™, BD Horizon™ V450408452Surface
CF™488A92350FITC, Alexa Fluor® 488490515Surface
CF™56892351Rhodamine Red, Alexa Fluor® 568562583Surface
CF™59492352Texas Red®, Alexa Fluor® 594593614Surface
CF™63392353Cy®5, Alexa Fluor® 633630650Surface
CF™640R92354Cy®5, Alexa Fluor® 647642662Surface
CF™64792359Cy®5, Alexa Fluor® 647650665Surface
CF™660C92360Alexa Fluor® 660667685Surface
CF™68092361Cy®5.5, Alexa Fluor® 660, IRDye®680LT681698Surface
CF™680R92355Cy®5.5, Alexa Fluor® 660, IRDye®680LT680701Surface
CF™40892356Pacific Blue™, BD Horizon™ V450408450Intracellular
CF™50092357FITC, Alexa Fluor® 488500510Intracellular
CF™54092358Tetramethylrhodamine (TAMRA), TRITC, Alexa Fluor® 546, Cy®3540570Intracellular
CF™55592364Alexa Fluor® 555, Cy®3, TRITC555585Intracellular
CF™65092363Cy®5, Alexa Fluor® 647650670Intracellular

Mix-n-Stain and CF dyes are covered by US and international patents, and are trademarks of Biotium, Inc. SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation; Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Invitrogen Corp; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Biosciences.

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Support & faq

Mix-n-Stain™ Antibody Labeling Kits

CF dyes are highly water soluble, small organic dyes designed by scientists at Biotium for labeling proteins and nucleic acids. With a series of over 20 colors (and growing), many of our CF dyes are brighter and more photostable than competing dyes. For more information please see the product flyers for individual CF dyes, the CF Dye Selection Guide, and our CF Dye FAQs.

Category: Mix-n-Stain™ Antibody Labeling Kits

← FAQs

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

 

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains, Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Apoptosis, Necrosis, and Cell Viability Kits, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

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← FAQs

Ask a question

CF™ Dyes

See our CF™ Dye Quick Reference Table for a list of dyes and summary of their features. Our CF™ Dye Selection Guide has more detailed information on each CF™ dye, and ordering information for our various CF™ dye product lines.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF™ Dyes

← FAQs

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

 

Category: GelRed™ and GelGreen™ Nucleic Acid Gel Stains, Mix-n-Stain™ Antibody Labeling Kits, DNA Quantitation Kits, NucView™ Caspase 3 Enzyme Substrates, Apoptosis, Necrosis, and Cell Viability Kits, Luciferase Assays, CF™ Dyes, Product shelf life, solubility, shipping and storage, and stability

← FAQs

CF initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF dyes based on cyanine dye structures. Since then, our CF dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF™ Dyes

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.