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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

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Product Description

Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW ~150 – 5000), high affinity biological ligands or substrates.

  • For SNAP-tag®, CLIP-tag™ and HaloTag®, or other ligands with an aliphatic amine
  • Label 100 nmol ligand
  • Reaction takes ~30 minutes with minimal hands-on time and no purification
  • Choose from 15 bright & photostable CF® Dyes
  • Cell membrane-permeant or impermeant options for intracellular or surface targets

For more information, please download the Mix-n-Stain™ Small Ligand Labeling Kit Flyer and the Product Information Sheet. We also offer TMP-PEG3-Amine for making fluorescent TMP ligands. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.

Mix-n-Stain™ Small Ligand Labeling Kits

DyeCatalog no.Spectrally similar dyesEx/Em (nm)Staining
CF®405M92362Pacific Blue™, BD Horizon™ V450408/452Surface
CF®40892356Pacific Blue™, BD Horizon™ V450408/450Intracellular
CF®488A92350FITC, Alexa Fluor® 488490/515Surface
CF®50092357FITC, Alexa Fluor® 488500/510Intracellular
CF®54092358TAMRA, TRITC, Alexa Fluor® 546, Cy®3540/570Intracellular
CF®55592364Alexa Fluor® 555, Cy®3, TRITC555/585Intracellular
CF®56892351Rhodamine Red, Alexa Fluor® 568562/583Surface
CF®59492352Texas Red®, Alexa Fluor® 594593/614Surface
CF®63392353Cy®5, Alexa Fluor® 633630/650Surface
CF®640R92354Cy®5, Alexa Fluor® 647642/662Surface
CF®64792359Cy®5, Alexa Fluor® 647650/665Surface
CF®65092363Cy®5, Alexa Fluor® 647650/670Intracellular
CF®660C92360Alexa Fluor® 660667/685Surface
CF®68092361Cy®5.5, Alexa Fluor® 660, IRDye®680LT681/698Surface
CF®680R92355Cy®5.5, Alexa Fluor® 660, IRDye®680LT680/701Surface
Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of Cytiva; IRDye is a registered trademark of LI-COR Biosciences.

Antibody & Protein Labeling Kits

Product
Name
Label
Options
Labeling
Scale
Labeling
Time
Features
Mix-n-Stain™ CF® Dye
Antibody Labeling Kits
CF® Dyes≤5-20 ug IgG
20-50 ug IgG
50-100 ug IgG
~ 15 min.• Rapid, simple labeling

• No purification

• Tolerates BSA & other additives

• Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC
Antibody Labeling Kits
FITC
Mix-n-Stain™ Cyanine Dye
Antibody Labeling Kits
Cyanine 555
Cyanine 647
Mix-n-Stain™ Biotin
Antibody Labeling Kits
Biotin
Mix-n-Stain™ Digoxigenin
Antibody Labeling Kits
Digoxygenin (DIG)
Mix-n-Stain™ DNP
Antibody Labeling Kits
DNP
Mix-n-Stain™ Maxi
Antibody Labeling Kits
CF® Dyes
Cyanine Dyes
1 mg IgG~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling KitsCF® Dyes
FITC
25 ug IgM
100 ug IgM
15-30 min.• Rapid, simple labeling

• No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling KitsCF® Dyes50 ug IgG~ 30 min.• Optimized to provide low 1-2.5 DOL

• No purification
Mix-n-Stain™
Nanobody Labeling Kits
CF® Dyes5-20 ug Nanobody®
20-50 ug Nanobody®
~ 30 min.• Optimized for Nanobodies®

• Tolerates BSA & other additives
Mix-n-Stain™
Small Ligand Labeling Kits
CF® Dyes0.1 umol
small ligand
~ 30 min.• Label SNAP®, CLIP™, HaloTag® & other small ligands

• Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo ImagingNear-IR CF® Dyes3 x 1 mg IgG~ 2 hours• Dyes, buffers, & spin vials for labeling + purification

• Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE
Protein Labeling Kits
CF® Dyes
Biotin
3 x 1 mg
protein
~ 2 hours• Dyes, buffers, & spin vials for labeling + purification
SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.

References

1. Sci Rep (2020) 10, 16686. https://doi.org/10.1038/s41598-020-73840-4

 

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