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Blood Group H Type-2 Monoclonal Mouse Antibody (19-OLE)

Recognizes the blood group H type 2 antigens, trisaccharide Fuc? ?1-2Gal? ?1-4GlcNAc? ?1 of human origin.

Product Attributes

Antibody number

0947

Reactivity (target)

Blood Group H Type-2

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

19-OLE

Isotype

IgM, kappa

Molecular weight

Multiple

Human gene symbol

ABO

Entrez gene ID

28

SwissProt

P16442

Unigene

654423

Immunogen

Mucinous colonic adenocarcinoma

Cellular localization

Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin)

Application notes

Immunohistology (formalin), Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

KG1 cells or human colorectal carcinoma.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Hematology

Conjugation
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Product Description

Recognizes the blood group H type 2 antigens, trisaccharide Fuc-a-1-2Gal1-4GlcNAc-a-1 of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

 

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