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Blood Group H Type 2 Monoclonal Mouse Antibody (19-OLE)

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Product Attributes

Antibody number

#0947

Antibody reactivity (target)

Blood Group H Type-2

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

19-OLE

Isotype

IgM, kappa

Molecular weight

Multiple

Synonyms

ABO Type O; Blood Group O; Blood Group H

Human gene symbol

ABO

Entrez gene ID

28

SwissProt

P16442

Unigene

654423

Immunogen

Mucinous colonic adenocarcinoma

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Plasma membrane

Species reactivity

Human

Antibody application notes

Immunohistology (formalin), Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

KG1 cells or human colorectal carcinoma.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cancer, Hematology

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-StainTM Antibody Labeling Kits for labeling antibodies with all CF® Dyes. We apologize for the inconvenience.
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Product Description

This antibody is applicable for staining ABO blood group O. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. This antibody recognizes the blood group H type 2 antigens, trisaccharide Fuc-a-1-2Gal1-4GlcNAc-a-1. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

 

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