It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
| Conjugation | Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP |
|---|---|
| Size | 50 uL, 100 uL, 250 uL, 500 uL |
| Supplied As | Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA |
| Antibody type | Primary |
| Host species | Mouse |
| Reactivity (target) | BrdU (Bromodeoxyuridine) (85-2C8) |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 85-2C8 |
| Molecular weight | Depends on the target |
| Synonyms | Bromodeoxyuridine, BUdr |
| Entrez gene ID | Not Applicable |
| SwissProt | Not Applicable |
| Unigene | Not Applicable |
| Immunogen | Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia) |
| Species reactivity | All species |
| Applications | Flow cytometry, Immunofluorescence |
| Cellular localization | Nuclear |
| Application notes | Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL |
| Positive control | Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU |
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Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.
