Monoclonal anti BrdU (Bromodeoxyuridine) (MoBu 1)

BNUB0884-0
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Catalog #: BNUB0884
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Product Description

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Harms et al. Acta Histochemica, Suppl. Band 36, 353-359 (1988). | Arras et al. J Clin Invest. 101(1), 40-50 (1998)

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

BrdU (Bromodeoxyuridine) (MoBu-1)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

MoBu-1

Molecular weight

Depends on the target

Synonyms

Bromodeoxyuridine, BUdr

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Unigene

Not Applicable

Immunogen

Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia)

Species reactivity

All species

Applications

Flow cytometry, Immunofluorescence

Cellular localization

Nuclear

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL

Positive control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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