It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
| Conjugation | Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP |
|---|---|
| Size | 50 uL, 100 uL, 250 uL, 500 uL |
| Supplied As | Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA |
| Antibody type | Primary |
| Host species | Mouse |
| Reactivity (target) | BrdU (Bromodeoxyuridine) (BRD469) |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | BRD469 |
| Molecular weight | 307Da |
| Synonyms | Bromodeoxyuridine, BUdr |
| Entrez gene ID | Not Applicable |
| SwissProt | Not Applicable |
| Unigene | Not Applicable |
| Immunogen | Bromodeoxyuridine (BrdU) conjugated to KLH |
| Species reactivity | All species |
| Applications | Flow cytometry, Immunofluorescence, Immunohistology (formalin) |
| Cellular localization | Nuclear |
| Application notes | Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT Use Ab at 1 ug/mL for 30 minute at RT |
| Positive control | Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU |
Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.
Expedited shipment may be requested at time of checkout.
Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.
