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BrdU Monoclonal Mouse Antibody (BRD469)

Product Attributes

Antibody number

#0469

Antibody reactivity (target)

BrdU

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

BRD469

Isotype

IgG1

Molecular weight

307Da

Synonyms

Bromodeoxyuridine; 5-bromo-2-deoxyuridine

Human gene symbol

Not Applicable

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Unigene

Not Applicable

Immunogen

Bromodeoxyuridine (BrdU) conjugated to KLH

Verified antibody applications

IHC (FFPE) (verified)

Antibody target cellular localization

Nucleus

Species reactivity

All species

Antibody application notes

Immunohistology formalin-fixed 0.5-1 ug/mL, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL

Positive control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cancer, Cell cycle

Primary Antibody Formats:

  • Purified: 200 ug/mL in PBS 0.05% BSA 0.05% azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no azide
  • Conjugation
    Don’t see what you’re looking for? Contact us for more conjugation options.
    Note: Please be advised that primary antibodies with catalog numbers that begin with “BN” will only be offered conjugated to CF®405S, CF®488A, CF®568, CF®594, CF®640R, CF®647, and biotin as of 5/27/2022. However, previously available primary antibody conjugates that are in stock will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with all CF® Dyes, fluorescent proteins, tandem dyes, and enzyme reporters. We apologize for the inconvenience.
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    Product Description

    Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA.

    This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).

     

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