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BrdU Monoclonal Mouse Antibody (BRD469)

This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU.

Product Attributes

Antibody number

0469

Reactivity (target)

BrdU

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

BRD469

Isotype

IgG1

Molecular weight

307Da

Synonyms

Bromodeoxyuridine, BUdr

Human gene symbol

Not Applicable

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Unigene

Not Applicable

Immunogen

Bromodeoxyuridine (BrdU) conjugated to KLH

Cellular localization

Nucleus

Species reactivity

All species

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 0.5-1 ug/mL, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL

Positive control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Cancer, Cell cycle

Primary Antibody Formats:

  • Purified: 200 ug/mL in PBS 0.05% BSA 0.05% azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no azide
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    Product Description

    This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

    References

    Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6. | Williams LS et. al. Cytometry, 1990, 11(4):490-7

     

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