Monoclonal anti BrdU (Bromodeoxyuridine) (BRD469 + BRD494 + BRD.3)

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Catalog #: BNUB1000
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Product Description

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.Williams LS et. al. Cytometry, 1990, 11(4):490-7

Additional Information


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

BrdU (Bromodeoxyuridine) (BRD469 + BRD494 + BRD.3)




BRD469 + BRD494 + BRD.3

Molecular weight

Depends on the target


Bromodeoxyuridine, BUdr

Entrez gene ID

Not Applicable


Not Applicable


Not Applicable


Bromodeoxyuridine (BrdU) conjugated to BSA or KLH

Species reactivity

All species


Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization


Application notes

Flow Cytometry 0.5-1 ug/million cells, Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/mL PBS, 10 min at 37oC

Positive control

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life