Polyclonal anti CD3e (Rabbit PAb)

BNUB0385-0
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Product Description

Recognizes the -chain of CD3, which consists of five different polypeptide chains (designated as γ, δ, ε, ζ, and η) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Cibull ML et. al. Histopathology, 1989, 15(6):599-605. | Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200

Additional Information

Applications

Flow cytometry, Immunofluorescence, Western

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Rabbit

Reactivity (target)

CD3e (Rabbit PAb)

Clonality

Polyclonal

Clone

Rabbit PAb

Isotype

IgG

Molecular weight

20 kDa

Entrez gene ID

916

SwissProt

P07766

Immunogen

A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e

Unigene

3003

Species reactivity

Human

Cellular localization

Cell surface and cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user, Predicted to show broad species reactivity, Western blotting 1-2 ug/mL for 60 minutes at RT

Positive control

Jurkat cells. Tonsil and lymph node

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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