Monoclonal anti CD59 (heavy chain of Protectin) (BRA 10G)

BNUB0181-0
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Product Description

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions.

Chorvath et al. Neoplasma 39(6),325-9 (1992) | Leukocyte Typing V (S F Schlossman, et?al, eds.) Oxford University Press,?Oxford (1995) p. 1476-1477. | M H Holguin, et al, (1996) J Immunol?157: 1659-1668. | A E Fritzinger, F.?Marciano-Cabral, et al. (2006)?Infection and Immunity?74(2):1189-1195. | J Zhang, C?Gerhardinger, M Lorenzi (2002) Diabetes?51(12): 3499-350

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP
Size

50 uL, 100 uL, 250 uL, 500 uL
Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA
Antibody type

Primary
Host species

Mouse
Reactivity (target)

CD59 (heavy chain of Protectin) (BRA-10G)
Clonality

Monoclonal
Isotype

IgG1, kappa
Clone

BRA-10G
Molecular weight

20 kDa
Synonyms
Entrez gene ID

966
SwissProt

P13987
Unigene

278573; 709466 & 710641
Immunogen

Human K562 tumor cells
Species reactivity

Human
Applications

Flow cytometry, Immunofluorescence
Cellular localization

Cell surface
Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control

Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life

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