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CD59 Monoclonal Mouse Antibody (MACIF/1193)

Reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein.

Product Attributes

Antibody number

1193

Reactivity (target)

CD59

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

MACIF/1193

Isotype

IgM, kappa

Molecular weight

20 kDa

Human gene symbol

CD59

Entrez gene ID

966

SwissProt

P13987

Unigene

278573; 709466 & 710641

Immunogen

Recombinant full-length human CD59 protein

Cellular localization

Membrane/cell surface

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin), Flow cytometry

Application notes

Immunohistology formalin-fixed 1-2 ug/mL, Does not react with baboon or horse, others not tested, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Research areas

Hematology, Immunology

Conjugation
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Product Description

Reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

This antibody is available purified with BSA/azide at 200 ug/mL, or BSA/azide-free at 1 mg/mL.

References

A E Fritzinger, F.?Marciano-Cabral, et al. (2006)?Infection and Immunity?74(2):1189-1195. | J Zhang, C?Gerhardinger, M Lorenzi (2002) Diabetes?51(12): 3499-3504 |

 

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