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CD63 Monoclonal Mouse Antibody (LAMP3/968)

Product Attributes

Antibody number

#0968

Antibody reactivity (target)

CD63

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

LAMP3/968

Isotype

IgG1, kappa

Molecular weight

26 kDa (core protein); 30-60 kDa (glycosylated)

Human gene symbol

CD63

Synonyms

gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30

Entrez gene ID

967

SwissProt

P08962

Unigene

445570

Immunogen

Recombinant human full-length CD63 protein

Antibody target cellular localization

Cytoplasmic, Exosomes/EVs

Species reactivity

Human

Verified antibody applications

Exosome staining (verified), Flow (surface) (verified), IF (verified), IHC (FFPE) (verified)

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology formalin-fixed 1-2 ug/mL, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user

Positive control

SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cancer, Exosomes/EVs, Immunology

Cell/tissue expression

Platelets, Granulocytes, Lymphocytes, Monocytes/macrophages

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Please be advised Biotium will no longer be selling primary antibodies conjugated to R-PE, APC, PerCP, AP, or HRP as of 4/18/22. However, certain protein-labeled primary antibodies will remain available until inventory is exhausted. Please view the list of available conjugates on the product page. We also recommend our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with these conjugates. We apologize for the inconvenience.
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Product Description

This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC05CF®405M408/452405DAPI (microscopy),
Pacific Blue™
CF®405M Features
BNC06CF®405L395/545405Pacific Orange™CF®405L Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC14CF®514516/548488GFP, FITCCF®514 Features
BNC43CF®543541/560532RFP, TRITCCF®543 Features
BNC55CF®555555/565532, 561RFP, TRITCCF®555 Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC60CF®660C667/685633-640Cy®5CF®660C Features
BNC61CF®660R663/682633-640Cy®5CF®660R Features
BNC80CF®680681/698633-640Cy®5.5, IR®700CF®680 Features
BNC81CF®680R680/701633-640Cy®5.5, IR®700CF®680R Features
BNC00CF®700695/720633-640AF700CF®700 Features
BNC70CF®770770/797785, 808IR®800CF®770 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free
N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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