Monoclonal anti Creatine Phosphokinase (2ba6)

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Catalog #: BNUB0196
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Product Description

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins

Mariman, E.C., et al. 1987. Structure and expression of the human creatine kinase B gene. Genomics 1: 126-137. | Mariman, E.C., et al. 1989. Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17: 6385

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Creatine Phosphokinase (CKBB/196)

Clonality

Monoclonal

Clone

2ba6

Isotype

IgG1, kappa

Molecular weight

43 kDa (Monomer); 86 kDa (Dimer)

Synonyms
Entrez gene ID

1152

SwissProt

P12277

Unigene

173724

Immunogen

Human CKBB protein

Species reactivity

Human

Applications

Immunohistology (frozen), Flow cytometry, Immunofluorescence

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology Frozen Only 0.5-1.0 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Predicted to show broad species reactivity

Positive control

Cerebellum

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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