Monoclonal anti Biotin (Hyb8)

BNUB0400-0
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Product Description

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Uccellini MB, et al. 2008. J. Immunol. 181:5875. | Green NM, et al. 2012. J Biol Chem. 287:39789 |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Biotin (Hyb8)

Clonality

Monoclonal

Isotype

IgG1

Clone

Hyb8

Molecular weight

244Da

Synonyms

Vitamin B7 or Vitamin H

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Unigene

Not Applicable

Immunogen

Biotinylated sheep immunoglobulin

Species reactivity

All species

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Depends on the target

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, In situ Nucleic Acid Hybridization, No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues Optimal dilution for a specific application should be determined by user, Western blotting

Positive control

Biotinylated proteins in solution or on tissues

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

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