Monoclonal anti Cytokeratin, HMW (34BE12)

BNUB0446-0
BNUB0446-1
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Catalog #: BNUB0446
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Product Description

This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58 | Varma M et. al. Mod Pathol 1999;12(5):472-8 |

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Cytokeratin, HMW (34BE12)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

34BE12

Molecular weight

50-67 kDa

Synonyms

KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

Entrez gene ID

51350

SwissProt

Q01546

Unigene

654392

Immunogen

Solubilized keratin extract from human stratum corneum

Species reactivity

Human, Mouse, Rat

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1.0 ug/mL, Immunohistology formalin-fixed 0.25-0.5 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1.0 ug/mL

Positive control

PC12 cells, Skin, Prostate carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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