Monoclonal anti Golgi Complex (371 4)

BNUB0102-0
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Product Description

This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Yuasa, K et. al. Binding and Phosphorylation of a Novel Male Germ Cell-specific cGMP-dependent Protein Kinase-anchoring Protein by cGMP-dependent Protein Kinase I. J Biol Chem, 275(7):4897-4905;2000. | Yoshio Endo et. al. Cellular localization and functional characterization of the equilibrative nucleoside transporters of antitumor nucleosides. Cancer science 98;2007

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Golgi Complex (371-4)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

371-4

Molecular weight

Not Known

Synonyms

Golgi Complex; Golgi apparatus; Golgi zone

Entrez gene ID

Not Known

SwissProt

Not Known

Unigene

Not Known

Immunogen

SU-DHL-1 large cell lymphoma cells.

Species reactivity

Human

Applications

Immunocytochemistry Acetone or paraformaldehyde fixed30 min, Flow cytometry, Immunofluorescence, Immunohistology (formalin), Western

Cellular localization

Golgi complex in cytoplasm

Application notes

Does not react with mouse or rat; others not known, Flow Cytometry 0.5-1.0 ug/million cells in 0.1 mL, Immunocytochemistry Acetone or paraformaldehyde fixed 0.5-1 ug/mL for 30 min, Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1.0 ug/mL

Positive control

HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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