Monoclonal anti Granulocyte Colony Stimulating Factor (G CSF) (CSF3) (CSF3/900)

BNUB0900-0
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Product Description

This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF’s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF’s share 73% sequence identity at the amino acid level.

Nagata, S., et al. 1986. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature 319: 415-418

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Granulocyte-Colony Stimulating Factor (G-CSF) (CSF3) (CSF3/900)

Clonality

Monoclonal

Isotype

IgM, kappa

Clone

CSF3/900

Molecular weight

19 kDa

Synonyms

Pluripoietin; Filgrastim; Lenograstim; CSF3OS; G-CSF; chromosome 17 open reading frame 33 (C17orf33); colony stimulating factor 3 (granulocyte)

Entrez gene ID

1440

SwissProt

P09919

Unigene

2233

Immunogen

Human recombinant full-length CSF3 protein

Species reactivity

Human, Macaque

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

HL60 cells. Tonsil or lymph node

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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