Monoclonal anti Granulocyte Marker (BM 1)

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Catalog #: BNUB0061

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Category: Primary Antibodies.

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Product Description

Recognizes 183kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.

Epstein AL; Samoszuk M; Stathopoulos E; Naeve GS; Clevenger CV; Weil S; Marder RJ. Immunohistochemical characterization of a 183 KD myeloid-specific-DNA-binding protein in B5 fixed, paraffin-embedded tissues, and bone marrow aspirates by monoclonal antibody BM-1. Blood, 1987, 70(4):1124-30

Additional Information

Conjugation	Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP
Size	50 uL, 100 uL, 250 uL, 500 uL
Supplied As	Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA
Antibody type	Primary
Host species	Mouse
Reactivity (target)	Granulocyte Marker (BM-1)
Clonality	Monoclonal
Isotype	IgG1
Clone	BM-1
Molecular weight	183 kDa
Synonyms	Myeloid specific protein; p183 DNA-binding cytoplasmic protein
Entrez gene ID	Not Known
SwissProt	Not Known
Unigene	Not Known
Immunogen	Human peripheral blood mononuclear cells
Species reactivity	Human
Applications	Flow cytometry, Immunofluorescence, Immunohistology (formalin)
Cellular localization	Cytoplasmic and nuclear
Application notes	Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, No special pretreatment is required for staining of formalin/paraffin tissues., Optimal dilution for a specific application should be determined by user
Positive control	HL60 cells. Bone marrow, lymph node or tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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