Monoclonal anti Human Nuclear Antigen (235 1)

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Catalog #: BNUB0099
 Multiple sizes
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Product Description

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Additional Information


Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP


50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type


Host species


Reactivity (target)

Human Nuclear Antigen (235-1)




IgG1, kappa



Molecular weight

70 kDa & 80 kDa



Entrez gene ID

Not Known


Not Known


Not Known


Nuclei of human myeloid leukemia biopsy cells

Species reactivity

Human, Non-human primates


Immunohistology (frozen), Immunoprecipitation, Flow cytometry, Immunofluorescence

Cellular localization


Application notes

Does not react with mouse, rat or chicken; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunocytochemistry Acetone-fixed cells 0.25-0.5 ug/mL for 30 minutes at RT, Immunofluorescence 0.5-1 ug/mL, Immunohistology Frozen 0.5-1.0 ug/mL for 30 minutes at RT, Immunoprecipitation 0.5-1 ug/500 ug protein lysate, Optimal dilution for a specific application should be determined by user

Positive control

All human cells. Tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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