Monoclonal anti Human Nuclear Antigen (235 1)

BNUB0099-0
BNUB0099-1BNUB0099-2
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Catalog #: BNUB0099
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Product Description

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Human Nuclear Antigen (235-1)

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone

235-1

Molecular weight

70 kDa & 80 kDa

Synonyms

HNA; HuNu

Entrez gene ID

Not Known

SwissProt

Not Known

Unigene

Not Known

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Species reactivity

Human, Non-human primates

Applications

Immunohistology (frozen), Immunoprecipitation, Flow cytometry, Immunofluorescence

Cellular localization

Nuclei

Application notes

Does not react with mouse, rat or chicken; others not known, Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunocytochemistry Acetone-fixed cells 0.25-0.5 ug/mL for 30 minutes at RT, Immunofluorescence 0.5-1 ug/mL, Immunohistology Frozen 0.5-1.0 ug/mL for 30 minutes at RT, Immunoprecipitation 0.5-1 ug/500 ug protein lysate, Optimal dilution for a specific application should be determined by user

Positive control

All human cells. Tonsil

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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