Monoclonal anti Lewis B (2 25LE)

Placeholder
string(39) "$120.00"
Price:
 from
  $120.00
Clear selection
Catalog #: BNUB1008
Selection:
 Multiple sizes
Category: .
>>> Order 5 or more and receive 10% off.

Product Description

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.

Richman, P.I. et al. 1987. Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization. Int. J. Cancer. 39: 317-328. Rouger, P.h., et al, eds. 1987. Proceedings of the first international work- shop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987). Blood Transfusion Immunohaematology 30: 353- 720. Bara, J. et al. 1988. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem. J. 254: 185-193. Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J. Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30.Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara. Alteration of sialyl Lewis epitope expression in pterygium. J.Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Lewis B (2-25LE)

Clonality

Monoclonal

Isotype

IgM, kappa

Clone

2-25LE

Molecular weight

45 kDa

Synonyms
Entrez gene ID

2525

SwissProt

P21217

Unigene

169238

Immunogen

Mucin isolated from a human ovarian cyst fluid

Species reactivity

Guinea Pig, Human, Pig

Applications

Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic

Application notes

Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Colon

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life