Monoclonal anti Lewis A (7LE)

BNUB0311-0
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Catalog #: BNUB0311
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Product Description

Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Blood transfusion and immunohaematology, Ph. Rouger, D Anstee and Ch. Salmon (Eds). Arnette, France 30 (5): 353-720 (1987). | Cancer Epidemiology, Biomarkers Prevention 1, 199-205 (1992). | Gastroenterology, 102 (2), 424-430 (1992)

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Lewis A (7LE)

Clonality

Monoclonal

Clone

7LE

Isotype

IgG1, kappa

Molecular weight

Multiple

Synonyms

Lewis A antigen

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Immunogen

Mucins isolated from ovarian cyst fluid

Unigene

Not Applicable

Species reactivity

Human, Mouse

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cell membrane, cytoplasmic and extracellular

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology (formalin), Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Colon tissue

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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