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Mitochondrial Marker Monoclonal Mouse Antibody (113-1)

Product Attributes

Antibody number

#0739

Reactivity (target)

Mitochondrial Marker

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

113-1

Isotype

IgG1, kappa

Molecular weight

60 kDa

Synonyms

Not Known

Entrez gene ID

Not Known

SwissProt

Not Known

Unigene

Not Known

Immunogen

Semi-purified mitochondrial preparation

Cellular localization

Mitochondria

Species reactivity

Human

Applications

Immunofluorescence, Immunohistology (formalin)

Application notes

Does not react with mouse or rat, others not known, Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes, Immunofluorescence (PFA- or MeOH-fixed) 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

HeLa or HepG2 cells. Hepatic carcinoma.

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Supplied As

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified, BSA-free: 1 mg/mL in PBS without azide, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide

Antibody research areas

Organelle markers

Conjugation
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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

This MAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 0739, Anti-Mitochondrial Marker (113-1) 

Antibody # prefixConjugationEx/Em (nm)Compatible Lasers (nm)Fluorescence detection channelsSupplied asSpecial applications/notes
BNC04CF®405S404/431405DAPI (microscopy), Alexa Fluor® 4050.1 mg/mL PBS, 0.1% BSA, 0.05% azideSIM
BNC05CF®405M408/452405DAPI (microscopy), Pacific Blue™0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM, STED, SIM, 2-Photon, TIRF
BNC06CF®405L395/545405Pacific Orange™0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSpectral cytometry/imaging
BNC88CF®488A490/515488GFP, FITC0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM, STED, 2-photon, TIRF
BNC14CF®514516/548488GFP, FITC0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSpectral cytometry/imaging
BNC43CF®543541/560532RFP, TRITC0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC55CF®555555/565532, 561RFP, TRITC0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM
BNC68CF®568562/583532, 561RFP, TRITC0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM, STED, SIM, TIRF
BNC94CF®594593/614561Texas Red®0.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM, STED, 2-photon
BNC40CF®640R642/662633-640Cy®50.1 mg/mL PBS, 0.1% BSA, 0.05% azideSIM, TIRF, and FLImP
BNC47CF®647650/665633-640Cy®50.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM
BNC60CF®660C667/685633-640Cy®50.1 mg/mL PBS, 0.1% BSA, 0.05% azideSpectral cytometry/imaging, STORM
BNC61CF®660R663/682633-640Cy®50.1 mg/mL PBS, 0.1% BSA, 0.05% azideHighly photostable
BNC80CF®680681/698633-640Cy®5.5, IR®7000.1 mg/mL PBS, 0.1% BSA, 0.05% azideSTORM, 3D SMLM, LI-COR® Odssey®
BNC81CF®680R680/701633-640Cy®5.5, IR®7000.1 mg/mL PBS, 0.1% BSA, 0.05% azide2-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC00CF®700695/720633-640Alexa Fluor® 7000.1 mg/mL PBS, 0.1% BSA, 0.05% azideSpectral cytometry/imaging
BNC70CF®770770/797785, 808IR®8000.1 mg/mL PBS, 0.1% BSA, 0.05% azideLI-COR® Odyssey®
BNCRR-PE (PE)496, 546, 565/578488-561PE0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAAPC650/660594-633APC0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCPPerCP482/677488-561PerCP, PE-Cy®50.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCBBiotinN/AN/AN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAPAlkaline PhosphataseN/AN/AN/A0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCHHorseradish PeroxidaseN/AN/AN/A0.1 mg/mL PBS, 0.05% BSA, no azide
BNUBPurified, with BSA N/AN/AN/A0.2 mg/mL PBS, 0.05% BSA, 0.05% azide
BNUMPurified, BSA-freeN/AN/AN/A1 mg/mL PBS, no BSA, no azide
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Tickoo SK, Amin MB, Linden MD, Lee MW, Zarbo RJ. Antimitochondrial antibody (113-1) in the differential diagnosis of granular renal cell tumors. Am J Surg Pathol. 1997;21(8):922-30.

Vera-Sempere F, Vera-Sirera B. Usefulness of antimitochondrial antibody 113-1 in diagnosis and classification of salivary gland tumours with oncocytic differentiation. Acta Otorrinolaringol Esp. 2011;62(1):1-9

 

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