Monoclonal anti Mucin 1 / EMA / Episialin / CD227 (E29)

BNUB0478-0
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Product Description

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Cordell J et al. 1985. Br J Cancer 52(3):347-54. | Heyderman E et al. 1985. Br J Cancer 52(3):355-61

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Mucin 1 / EMA / Episialin / CD227 (E29)

Clonality

Monoclonal

Clone

E29

Isotype

IgG2a, kappa

Molecular weight

265-400 kDa

Synonyms
Entrez gene ID

4582

SwissProt

P15941

Immunogen

Delipidated extract of human milk fat globule membranes

Unigene

89603

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasmic & cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.1-0.2 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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