Monoclonal anti Mucin 1 / EMA / Episialin / CD227 (MUC1/520)

BNUB5520-0
BNUB5520-1BNUB5520-2BNUB5520-3BNUB5520-4BNUB5520-5
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Product Description

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Stanley CM, Phillips TE. Am J Physiol. 1999;277:G191-200

Additional Information

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Mucin 1 / EMA / Episialin / CD227 (MUC1/520)

Clonality

Monoclonal

Clone

MUC1/520

Isotype

IgG, kappa

Molecular weight

265-400 kDa

Entrez gene ID

4582

SwissProt

P15941

Unigene

89603

Immunogen

Human milk-fat globule membranes (HMFGM)

Species reactivity

Human

Cellular localization

Cytoplasmic & cell surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.1-0.2 ug/mL for 30 min at RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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