Monoclonal anti pgp9.5 (UCHL 1) (13C4)

BNUB0147-0
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Product Description

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson’s disease.

Day IN et. al. Biochem Society Trans 14:350-351 (1986

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

pgp9.5 (UCHL-1) (13C4)

Clonality

Monoclonal

Clone

13C4

Isotype

IgG2a, kappa

Molecular weight

20-30 kDa

Synonyms
Entrez gene ID

7345

SwissProt

P09936

Immunogen

Native UchL1 (PGP9.5) protein from brain

Unigene

518731

Species reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunofluorescence, Western

Cellular localization

Cytoplasmic. Endoplasmic Reticulum membrane

Application notes

Immunofluorescence 1-2 ug/mL, Optimal dilution for a specific application should be determined by user, Western blotting 0.5-1 ug/mL

Positive control

Cerebellum

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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