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PGP9.5 / UchL1 Monoclonal Mouse Antibody (UCHL1/775)

Product Attributes

Antibody number

#0775

Reactivity (target)

PGP9.5, UchL1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

UCHL1/775

Isotype

IgG1, kappa

Molecular weight

20-30 kDa

Synonyms

Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1

Human gene symbol

UCHL1

Entrez gene ID

7345

SwissProt

P09936

Unigene

518731

Immunogen

Recombinant human UCHL1 protein

Verified antibody applications

IHC (FFPE) (verified), WB (verified)

Antibody target cellular localization

Cytoplasmic, Endoplasmic reticulum

Species reactivity

Human, Rat

Antibody application notes

Immunohistology formalin-fixed 1-2 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL, Predicted to show broad species reactivity, Optimal dilution for a specific application should be determined by user

Positive control

Cerebellum

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate buffer formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Metabolism, Neuroscience

Cell/tissue expression

Neurons

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson’s disease.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0775, Anti-PGP9.5 (UCHL1/775) 

Antibody # prefixConjugationEx/Em (nm)Compatible Lasers (nm)Fluorescence detection channelsSpecial applications/notes
BNC04CF®405S404/431405DAPI (microscopy), Alexa Fluor® 405SIM
BNC05CF®405M408/452405DAPI (microscopy), Pacific Blue™STORM, STED, SIM, 2-Photon, TIRF
BNC06CF®405L395/545405Pacific Orange™Spectral cytometry/imaging
BNC88CF®488A490/515488GFP, FITCSTORM, STED, 2-photon, TIRF
BNC14CF®514516/548488GFP, FITCSpectral cytometry/imaging
BNC43CF®543541/560532RFP, TRITC
BNC55CF®555555/565532, 561RFP, TRITCSTORM
BNC68CF®568562/583532, 561RFP, TRITCSTORM, STED, SIM, TIRF
BNC94CF®594593/614561Texas Red®STORM, STED, 2-photon
BNC40CF®640R642/662633-640Cy®5SIM, TIRF, and FLImP
BNC47CF®647650/665633-640Cy®5STORM
BNC60CF®660C667/685633-640Cy®5Spectral cytometry/imaging, STORM
BNC61CF®660R663/682633-640Cy®5Highly photostable
BNC80CF®680681/698633-640Cy®5.5, IR®700STORM, 3D SMLM, LI-COR® Odssey®
BNC81CF®680R680/701633-640Cy®5.5, IR®7002-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC00CF®700695/720633-640Alexa Fluor® 700Spectral cytometry/imaging
BNC70CF®770770/797785, 808IR®800LI-COR® Odyssey®
BNCRR-PE (PE)496, 546, 565/578488-561PE
BNCAAPC650/660594-633APC
BNCPPerCP482/677488-561PerCP, PE-Cy®5
BNCBBiotinN/AN/AN/A
BNCAPAlkaline PhosphataseN/AN/AN/A
BNCHHorseradish PeroxidaseN/AN/AN/A
BNUBPurified, with BSA N/AN/AN/A
BNUMPurified, BSA-freeN/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of GE Healthcare; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
 

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