Monoclonal anti Phosphotyrosine (PY20)

BNUB0232-0
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Product Description

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Glenny, Jr. JR, et. al. Journal of Immunological Methods, 1988, 109:277-85

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

Phosphotyrosine (PY20)

Clonality

Monoclonal

Clone

PY20

Isotype

IgG2b

Molecular weight

Depends upon the phosphorylated target

Synonyms

P-Tyr

Entrez gene ID

Not Applicable

SwissProt

Not Applicable

Immunogen

Phosphotyrosine conjugated to KLH

Unigene

Not Applicable

Species reactivity

All species

Applications

Immunoprecipitation, Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Depends upon the location of phosphorylated target

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), Immunofluorescence 1-2 ug/mL, Immunohistology formalin-fixed 1-2 ug/mL for 30 minutes at RT, Immunoprecipitation 1-2 ug/500 ug protein lysate, No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues, Optimal dilution for a specific application should be determined by user, Western blotting 0.5-1 ug/mL

Positive control

MCF-7, MDA-231, T47-D cells or breast carcinoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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