Monoclonal anti S100A9 (47-8D3)

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Catalog #: BNUB1093
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Product Description

This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer’s cells of the liver and Langerhan’s cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Flavell DJ. et al., J. Histochem. Cytochem. 35, 12171226, 1987. | Facchetti F. et al., Am. J. Clin. Pathol. 92, 4250, 1989. | Bardadin KA. et al., J. Pathol. 164, 253259, 1991. | Goebeler M. Et al., J. Leukocyte Biol. 55, 259-261, 1994

Additional Information

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

S100A9 (47-8D3)

Clonality

Monoclonal

Isotype

IgM

Clone

47-8D3

Molecular weight

14 kDa

Entrez gene ID

6280

SwissProt

P06702

Immunogen

Human peripheral blood monocyte components

Unigene

112405

Species reactivity

Human, Rat

Cellular localization

Cytoplasmic, Extracellular (secreted) and Cell Surface

Application notes

Flow Cytometry (0.5-1 ug/million cells/0.1 mL), For staining of formalin-fixed, paraffin-embedded tissues, digest with trypsin at 1mg/mL PBS, 15 min at RT, Immunofluorescence 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1 ug/mL for 30 minutes at RT

Positive control

Liver or histiocytoma

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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