NBD-Ethylenediamine
The fluorescence of NBD is highly dependent on the environment; it is virtually nonfluorescent in water but becomes strongly green fluorescent in hydrophobic media. NBD-ethylenediamine can be readily coupled to activated carboxy groups or sulfonyl chlorides.
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Product Description
The fluorescence of NBD is highly dependent on the environment; it is virtually nonfluorescent in water but becomes strongly green fluorescent in hydrophobic media. NBD-ethylenediamine can be readily coupled to activated carboxy groups or sulfonyl chlorides.
- λEx/λEm (MeOH) = 466/535 nm
- Yellow solid soluble in DMSO or DMF
- Store at 4°C and protect from light
- C8H9N5O3
- MW: 223.19
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NBD C6-Sphingomyelin
NBD C6-sphingomyelin (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosyl phosphocholine) has been used for following sphingolipid metabolism in cells.
NBD C6-Ceramide
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NBD Chloride
NBD chloride (4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole) is nonfluorescent until it reacts with primary or secondary amines to produce a fluorescent product. NBD chloride has been extensively used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines.
EDC (EDAC)
EDC (or EDAC) (1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, hydrochloride)) has recently been found to be very useful for fixing in situ chelators, including the fluorescent ion indicators for histological studies following physiological experiments. EDC is also a widely used reagent to activate carboxy groups for amine coupling.
DMSO, Anhydrous
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.