Peroxidase, Galactosidase, Phosphatase, & Other Substrates

The sampler kit contains 10 mg each of BCIP Na (10001), BCIP Pink (10006), BCIP Red (10004) and 250 mg of NBT (10008). NBT forms a co-precipitant and enhances the BCIP reaction.

For researchers wishing to investigate the suitability of our various chromogenic β-galactosidase substrates, we offer this sampler kit, which contains 5 mg each of the following five different substrates: X-Gal (10011), Red-β-D-Gal (10012), Rose-β-D-Gal (10013), Purple-β-D-Gal (10014) and Green-β-D-Gal (10015).

XTT (2,3-Bis-(2-Methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, disodium salt) is a tetrazolium derivative used widely in cell viability and proliferation testing. The material is readily reduced in viable cells to a highly water-soluble orange colored product (λ= 475 nm).

10-Acetyl-3,7-dihydroxyphenoxazine (Amplex® Red), is regarded as the best fluorogenic substrate for peroxidase and H₂O₂ because it is highly sensitive, specific and stable.

Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes.

Dihydrorhodamine 123 is the reduced form of rhodamine 123 (70010), which is a commonly used fluorescent mitochondrial dye.

Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 (10055) but with increased stability toward air oxidation and light during storage.

A red derivative of BCIP that produces a red (λ_max 565 nm) colored precipitate instead of the blue/violet precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins and are compatible with a variety of applications.

A red derivative of BCIP that produces a red (λ_max 565 nm) colored precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications.