Dyes For Labeling Carbonyls & Carbohydrates
Aminooxy reagents (also known as hydroxylamine or aminooxyacetamide) react with aldehydes or ketones to form a stable oxime bond under mild conditions without the use of a reducing agent. Aminooxy can be used to label abasic sites in damaged DNA or carbonyl groups resulting from protein oxidation. They also can be used to label carbohydrates or glycoproteins after oxidation of the carbohydrates to form carbonyl groups. The reaction is fast and can be further accelerated by adding aniline as a catalyst. Aminooxy reagents are superior labeling reagents to hydrazides, because they form more stable linkages.
Hydrazides also react with aldehydes or ketones, but form unstable hydrazone linkages. Hydrazides are non-toxic and highly water soluble, making them useful as fluid phase markers and fixable microinjectable cytoplasmic tracers.
Fluorescent CF® Dye Aminooxy and CF® Dye Hydrazide are available with a wide selection of bright and photostable colors. For 2D DIGE platform, we offer CF®DI reactive dyes, which have matched molecular weight and charge. We also provide a wide selection of classic dyes and biotin in aminooxy and hydrazide forms.
CF® Dye Aminooxy
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CF® dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharides, glycoproteins or antibodies.
CF® Dye Hydrazide
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Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
CF®DI Aminooxy
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CF®DI Aminooxy derivatives are useful for fluorescently labeling aldehyde/ketone groups on biomolecules such as polysaccharides, glycoproteins or antibodies to generate size- and charge-matched conjugates.
CF®DI Hydrazide
92164, 92165, 92166, 92184, - 92185View allHide
CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).
Cyanine Dye Alkyne
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Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.
Cyanine Dye Aminooxy
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Cyanine dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.
Aminooxy-5(6)-FAM
Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.
Aminooxy-5(6)-ROX
Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.
Aminooxy-5(6)-TAMRA
Aminooxy-5(6)-TAMRA reacts with aldehydes or ketones to form a stable oxime linkage under mild conditions. It can also be used to label abasic sites in damaged DNA.
Aniline, 10X in Acetate Buffer
Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.
ANTS
ANTS (8-aminonaphthalene-1,3,6-trisulfonic acid, disodium salt) is a highly negatively charged dye with an amino group that can be coupled to an aldehyde or ketone group to form an unstable Schiff base. The Schiff base is usually chemically reduced by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaB(CN)H3) to form a stable linkage (1).
APTS
APTS is a very useful green fluorescent dye for labeling glycoproteins or sugar molecules in general. The labeling occurs via reductive amination which involves the formation of a Schiff base between the amine of APTS and the aldehyde or ketone of the sugar, followed by the reduction of the Schiff base linkage to a stable carbon-nitrogen bond.
ARP
ARP (N-(Aminooxyacetyl)-N’-(D-biotinoyl)hydrazine, trifluoroacetic acid salt) reacts with the exposed aldehyde group formed at abasic sites in damaged DNA, allowing the DNA to be labeled with biotin groups (1,2). The labeled DNA can then be quantitated with fluorescent or enzyme-conjugated streptavidin complexes.