Cyanine Dye Alkyne
Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.
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CF® Dye Azide
CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.
CF® Dye Picolyl Azide
CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.
CF® Dye BCN
CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.