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Cyanine Dye Alkyne

Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

Product Attributes

Dye Color

Cyanine 555, Cyanine 647

Chemical reactivity (reacts with)

Azides/Picolyl azides

Functional group

Alkyne

Storage Conditions

Store at -10 to -35 °C, Protect from light

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Product Description

Cyanine 555 and Cyanine 647 alkynes can be used to analyze O-GlcNAcylated proteins by 2D glyco-DIGE (differential gel electrophoresis). They react with azide groups to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. Cyanine 555 and Cyanine 647 are structurally identically to Cy®3 and Cy®5 dyes respectively from GE Healthcare.

  • Size- and charge-matched for analysis of O-GlcNAcylated proteins via 2D glyco-DIGE.
  • React with azides to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.
  • Structurally identical to Cy®3 and Cy®5 dyes.

Cyanine 555 (aka Cy®3):

    • λExEm (water) = 555/565 nm
    • Dark red solid soluble in water, DMF and DMSO
    • C40H56N4O7S2
    • MW: 769

Cyanine 647 (aka Cy®5):

    • λExEm (water) = 650/665 nm
    • Dark blue solid soluble in water, DMF and DMSO
    • C42H58N4O7S2
    • MW: 795

Cy3 and Cy5 are registered trademarks of GE Healthcare.

Also see CF® Dye Alkynes, available with a wide selection of our bright and photostable CF® dyes.

 

 

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