Support & Resources

+1

Cyanine Dye Alkyne

Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.

Product Attributes

Chemical reactivity (reacts with)

Azides/Picolyl azides

Functional group

Alkyne

Storage Conditions

Store at -10 to -35 °C, Protect from light

Learn more about

Technology

Conjugation
Don’t see what you’re looking for? Contact us for more conjugation options.
Size
Catalog #
price
Qty
1 mg
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Product Description

Cyanine 555 and Cyanine 647 alkynes can be used to analyze O-GlcNAcylated proteins by 2D glyco-DIGE (differential gel electrophoresis). They react with azide groups to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

  • Size- and charge-matched for analysis of O-GlcNAcylated proteins via 2D glyco-DIGE.
  • React with azides to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Cyanine 555:

    • λExEm (water) = 555/565 nm
    • Dark red solid soluble in water, DMF and DMSO
    • C40H56N4O7S2
    • MW: 769

Cyanine 647:

    • λExEm (water) = 650/665 nm
    • Dark blue solid soluble in water, DMF and DMSO
    • C42H58N4O7S2
    • MW: 795

Also see CF® Dye Alkynes, available with a wide selection of our bright and photostable CF® dyes.

 

 

You may also like…

    CF® Dye Azide

    CF® Dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.

    CF® Dye BCN

    CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labeling reactions are also available.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    CF® Dye Alkyne

    CF® Dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.