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Cyanine Dye Alkyne

Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform. Cyanine 555 is structurally identical to Cy®3, and Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.

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Cyanine 555, Cyanine 647

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Product Description

Both cyanine 555 alkyne and cyanine 647 alkyne are used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform (1).

Cyanine 555 is structurally identical to Cy®3 from GE Healthcare. Cyanine 555 alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

  • λExEm (water) = 555/565 nm
  • Dark red solid soluble in water, DMF and DMSO
  • Store at -20°C and protect from moisture and light
  • C40H56N4O7S2
  • MW: 769

Cy3 is a registered trademark of GE Healthcare.

Cyanine 647 is structurally identical to Cy®5 from GE Healthcare. Cyanine 647 alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. Both cyanine 647 alkyne and cyanine 555 alkyne have been used to analyze O-GlcNAcylated proteins in “2D glyco-DIGE” platform (1).

  • λExEm (water) = 650/665 nm
  • Dark blue solid soluble in water, DMF and DMSO
  • Store at -20°C and protect from moisture and light
  • C42H58N4O7S2
  • MW: 795

Also see CF® Dye Alkynes, available with a wide selection of our bright and photostable CF® dyes.

Cy3 and Cy5 are registered trademarks of GE Healthcare.

References

1. Cell Reports 5, 546-552 (2013)

 

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