Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

Fluorescein tyramide can be used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Aniline, 10X in acetate buffer is designed to use as catalytic reagent for the optimal coupling of protein carbonyls with aminooxy compounds.

Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.

CBQCA (3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) reacts specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. CBQCA also reacts with hydrophilic peptides and amino sugars.

Biotin-XX tyramide conjugate can be used for tyramide signal amplification (TSA) to generate high-density labeling of a target protein or nucleic acid with biotin.

Biotin azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Biotin alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition.

Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides.

5-CR110-PEO₃-amine has a long water-soluble PEG spacer that separates the green fluorescent CR110 and the amine functional group.

Aminooxy-5(6)-ROX reacts with aldehydes or ketones to a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.

Aminooxy-5(6)-TAMRA reacts with aldehydes or ketones to form a stable oxime linkage under mild conditions. It can also be used to label abasic sites in damaged DNA.

Our aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable oxime linkage under mild conditions, and can be used to label abasic sites in damaged DNA.

SR101-PEO₁₂ SE (Sulforhodamine 101-PEO₁₂-propionic acid succinimydyl ester; Texas Red®-PEO₁₂ SE) has an approximately 47 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.

SR101-PEO₈ SE (Sulforhodamine 101-PEO₈-propionic acid succinimydyl ester; Texas Red®-PEO₈ SE) has an approximately 32 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.

5(6)-ROX maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement of 5(6)-carboxy-X-rhodamine C5-maleimide (91041).