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Biotin-XX Tyramide

This biotin-XX  tyramide conjugate can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ.

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Product Description

This  biotin-XX  tyramide conjugate can be used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal.  The deposited biotin molecules can be detected with any of our CF® dye-labeled streptavidin conjugates.

Also see our CF® Dye Tyramide Signal Amplification Kits.

References

1. Journal of Immunological Methods 125, 279-285 (1989).

2. Journal of Immunological Methods 137, 103-112 (1991).

3. Cytometry A. (2010), doi:10.1002/cyto.a.20970

 

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