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Fluorescent CF® Dyes CF®488A Dye

CF®488A Dye

A superior green fluorescent dye
  • Minimally charged, for more specific conjugates
  • Better separation from the red channel
  • Validated in STED and TIRF super-resolution imaging
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

CF®488A is a green fluorescent dye for the 488 nm argon laser line. CF®488A is at least as bright as Alexa Fluor® 488. However, Alexa Fluor® 488 carries multiple negative charges, which can significantly alter antibody specificity. CF®488A is minimally charged, for high specificity. CF®488A also has a shorter emission wavelength than Alexa Fluor® 488 and FITC, for less fluorescence spill-over into the red channel. CF®488A has been validated in STED and TIRF super-resolution imaging methods (learn more about CF® Dyes for Super-Resolution Imaging).

See products available with CF®488A. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

Figure 1. Normalized absorption and emission spectra of CF®488A goat anti-mouse in PBS.

CF®488A Technical Summary

  • Abs/Em maxima: 490/515 nm
  • Extinction coefficient: 70,000
  • Molecular weight: ~914
  • Excitation laser line: 488 nm
  • Alternative for: Alexa Fluor® 488, Cy®2, DyLight® 488, fluorescein (FITC)
  • Fluorescence lifetime:
  • τ (ns)/free acid in PBS pH 7.4, ε (ns) = 4.11 ± 0.05
  • τ (ns) /S.Ab§ = 1.705

Visit our FAQ for more fluorescence life time information on CF® Dyes.

Figure 2. CF®488A is much more photostable than FITC. HeLa cells were stained with CF®488A phalloidin or FITC phalloidin and imaged at time zero, or after 5 sec... See More
Figure 3. HeLa cells stained with rabbit anti-COX IV and CF®488A goat anti-rabbit (mitochondria, green), and mouse anti-Golgin 97 and CF®555 goat anti-mouse (Go... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®450 Dye
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CF®503R Dye

CF is a registered trademark of Biotium. Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.

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