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CF™660C and CF™660R Dyes

CF™660C and CF™660R are two spectrally similar fluorescent dyes that emit fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR (Figure 1). Although their absorption maxima are at around 660 nm, both dyes can be sufficiently excited by the 633 or 635 nm laser. When combined with other CF™ dyes of shorter wavelengths, CF™660C or CF™660R can serve as a useful long wavelength dye in multi-color detection applications. The two dyes are spectrally similar to Alexa Fluor® 660 but are far superior to the latter in performance.

Like Alexa Fluor® 660, CF™660C is a cyanine-based dye. However, when conjugated to protein, CF™660C is several fold brighter and significantly more photostable than Alexa Fluor® 660 (Figures 2 and 3). CF™660R is a rhodamine-based dye. Like rhodamine dyes in general, CF™660R is exceptionally photostable, compared to both Alexa Fluor® 660 and CF™660C (Figure 2). CF™660R is also much brighter than Alexa Fluor® 660, though not as bright as CF™660C (Figure 2). The superior photostability and excellent brightness of CF™660R make the dye an ideal choice for confocal microscopy and other demanding applications.

A full selection of reactive dyes, secondary antibodies, antibody labeling kits, and other bioconjugates including phalloidins, Annexin V and α-bungarotoxin are available for CF™ dyes.

View products associated with this technology…

CF™660C and CF™660R conjugates, labeling kits, and reactive dyes

Technical Summary

CF™660C

  • Abs/Em Maxima: 667/685 nm
  • Extinction coefficient: 200,000
  • Molecular weight: ~3112
  • Flow cytometry laser line: 633 or 635 nm
  • Microscopy laser line: 633 or 635 nm
  • Direct replacement for: Alexa Fluor® 660, Allophycocyanin (APC)

CF™660R

  • Abs/Em Maxima: 663/682 nm
  • Extinction coefficient: 100,000
  • Molecular weight: ~888
  • Flow cytometry laser line: 633 or 635 nm
  • Microscopy laser line: 633 or 635 nm
  • Direct replacement for: Alexa Fluor® 660, Allophycocyanin (APC)

Advantages of CF™660C:

  • Much brighter than Alexa Fluor® 660
  • More photostable than Alexa Fluor® 660
  • Highly water-soluble

Advantages of CF™660R:

  • Brighter than Alexa Fluor® 660
  • The most photostable 660 nm dye ideal for confocal microscopy applications
  • Highly water-soluble
Figure 1. Absorption and emission spectra of CF™660C and CF™660R conjugated to goat anti-mouse IgG, respectively, in PBS.
Figure 1. Absorption and emission spectra of CF™660C and CF™660R conjugated to goat anti-mouse IgG, respectively, in PBS.
Figure 2. Relative fluorescence of CF™660C-, CF™660R and Alexa Fluor® 660-goat anti-mouse conjugates as a function of number of dye per protein (i.e., degree of labeling). Fluorescence was measured at each dye’s emission maximum in PBS using 633 nm excitation.
Figure 2. Relative fluorescence of CF™660C-, CF™660R and Alexa Fluor® 660-goat anti-mouse conjugates as a function of number of dye per protein (i.e., degree of labeling). Fluorescence was measured at each dye’s emission maximum in PBS using 633 nm excitation.
Figure 3. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™660C (Biotium) or Alexa Fluor® 660 (Invitrogen) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0 and 10 second.
Figure 3. Jurkat cells were fixed, permeabilized and stained with rabbit anti-CD3 (Abcam) followed by CF™660C (Biotium) or Alexa Fluor® 660 (Invitrogen) goat anti-rabbit IgG conjugates. Cells were imaged using an Olympus mercury arc lamp microscope equipped with a Cy5 filter set and CCD camera. Images were taken at t=0 and 10 second.