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CF™620R Dye

CF™620R dye is a far-red rhodamine-based fluorescent dye. The dye is highly fluorescent and extremely photostable. With its absorption and emission maxima centered at 617 and 639 nm, respectively, the dye may be used as an excellent energy acceptor in fluorescence resonance energy transfer (FRET), or used in multi-color detection applications where the excitation and emission windows can be matched with the spectral profiles of the dye for maximal fluorescence collection. The exceptional water solubility of the dye facilitates bioconjugation in aqueous media and better retain the biological specificity of the conjugates.

Two reactive forms of CF™620R dye are currently available. The succinimidyl ester dye is for labeling amino molecules while the maleimide dye is for labeling molecules with a thiol group.

Technical Summary

  • Abs/Em Maxima: 617/639 nm
  • Extinction coefficient: 115,000
  • Molecular weight: 738
  • Direct replacement for: LightCycler® Red 640 (LC640)

Advantages

  • Highly water-soluble
  • Highly fluorescent
  • Extremely photostable
Figure 1. Absorption and emission spectra of CF™620R dye in PBS.
Figure 1. Absorption and emission spectra of CF™620R dye in PBS.