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CF®620R Dye

A Unique Far-Red Dye

Bright and photostable
Highly water soluble
Unique FRET acceptor dye

Figure 1. Normalized absorption and emission of CF®620R free acid in PBS.

CF®620R dye is a far-red rhodamine-based fluorescent dye. The dye is highly fluorescent and extremely photostable. With its absorption and emission maxima centered at 617 and 639 nm, respectively, the dye may be used as an excellent energy acceptor in fluorescence resonance energy transfer (FRET), or used in multi-color detection applications with instruments that match the unique spectral profiles of the dye for maximal signal. The exceptional water solubility of the dye facilitates bioconjugation and better preserves biological specificity of the conjugates. We offer amine-reactive and thiol-reactive forms of CF®620R (see below). Additional reactive dyes or conjugates may be added on request, contact techsupport@biotium.com to inquire.

For bright and photostable far-red dyes for microscopy or flow cytometry, see our CF®633 and CF®640R dyes.

CF®620R Technical Summary

  • Abs/Em maxima: 617/639 nm
  • Extinction coefficient: 115,000
  • Molecular weight: ~738
  • Laser line: 633 nm or 635 nm
  • Spectrally similar to: LightCycler® Red 640 (LC640)

CF®594ST Dye < Previous           CF® Dyes Overview           Next > CF®633 Dye

CF Dye Succinimidyl Ester

From: $147 Sizes: 0.25 umol, 1 umolCatalog #: 92137, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, 92105, 92104, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, - 92120View allHide

CF Dye Maleimide

From: $184 Sizes: 0.25 umol, 1 umolCatalog #: 92020, 92033, 92034, 92044, 92045, 92046, 92118, 92124, 92128, 96012, 96015, 92032, 92031, 92021, 92022, 92023, 92024, 92025, 92026, 92027, 92028, 92029, 92030, - 96017View allHide

Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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