CF®532 is an orange colored fluorescent dye with absorption and emission maxima at 527 and 558 nm, respectively (Figure 1). The dye is based on a new class of rhodamine dyes invented by Biotium scientists. Unlike traditional rhodamine dyes, CF®532 is not only bright and photostable but also highly water-soluble. As a result, CF®532 antibody conjugates retain excellent solubility and yield the brightest fluorescence among antibody conjugates prepared from spectrally similar dyes like Alexa Fluor® 532 (Figures 2, 3). See CF®532 product lines below.
CF®532 Technical Summary
- Abs/Em maxima: 527/558 nm
- Extinction coefficient: 96,000
- Molecular weight: ~685
- Laser line: 532 nm
- Direct replacement for: Alexa Fluor® 532, Atto 532
CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific. FACSCalibur is a trademark of BD Biosciences.