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CF®532 Dye

Bright orange dye for the 532 nm laser

Designed for the 532 nm laser
Brighter than Alexa Fluor® 532
Water soluble & pH-insensitive

Figure 1. Normalized absorption and emission spectra of CF®532 goat anti-mouse conjugate in PBS.

CF®532 is an orange colored fluorescent dye with absorption and emission maxima at 527 and 558 nm, respectively (Figure 1). The dye is based on a new class of rhodamine dyes invented by Biotium scientists. Unlike traditional rhodamine dyes, CF®532 is not only bright and photostable but also highly water-soluble. As a result, CF®532 antibody conjugates retain excellent solubility and yield the brightest fluorescence among antibody conjugates prepared from spectrally similar dyes like Alexa Fluor® 532 (Figures 2, 3). See CF®532 product lines below.

CF®532 Technical Summary

  • Abs/Em maxima: 527/558 nm
  • Extinction coefficient: 96,000
  • Molecular weight: ~685
  • Laser line: 532 nm
  • Direct replacement for: Alexa Fluor® 532, Atto 532

Figure 2. CF®532 generates brighter fluorescent conjugates than Alexa Fluor® 532. Relative fluorescence of goat anti-mouse conjugates with varying degrees of labeling (DOL, or dye molecules per antibody).
Figure 3. CF®532 gives brighter staining than Alexa Fluor® 532. Jurkat cells were stained with mouse anti-CD3 antibody, followed by goat anti-mouse conjugates with varying degrees of labeling (DOL, or dye molecules per antibody). Geometric mean fluorescence was analyzed on a BD FACSCalibur™ flow cytometer in the FL2 channel.

CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific. FACSCalibur is a trademark of BD Biosciences.

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Phalloidin Conjugates

From: $75 Sizes: 100 U, 50 U, 300 UCatalog #: 00027, 00055, 00034-T, 00040-T, 00041-T, 00042-T, 00043-T, 00044-T, 00045-T, 00046-T, 00047-T, 00048-T, 00049-T, 00050-T, 00051-T, 00052-T, 00053-T, 00054-T, 00054, 00053, 00052, 00028, 00030, 00032, 00033, 00034, 00040, 00041, 00042, 00043, 00044, 00045, 00046, 00047, 00048, 00049, 00050, 00051, - 00055-TView allHide

Streptavidin Conjugates

From: $93 Sizes: 100 uL, 200 uL, 1 mL, 1 mgCatalog #: 29030, 29056, 29065, 29066, 29072, 29044-1mL, 29044-200uL, 29048-1mL, 29048-200uL, 29071-100uL, 29071-1mL, 29049, 29043, 29041, 29031, 29032, 29033, 29034, 29035, 29036, 29037, 29038, 29039, 29040, - 29081View allHide

CF Dye & Biotin SE Protein Labeling Kits

From: $383 Sizes: 3 labelingsCatalog #: 92208, 92219, 92220, 92221, 92222, 92223, 92224, 92225, 92226, 92218, 92217, 92209, 92210, 92211, 92212, 92213, 92214, 92215, 92216, - 92228View allHide

Mix-n-Stain CF Dye Antibody Labeling Kits

From: $95 Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92333, 92285, 92284, 92283, 92282, 92281, 92280, 92279, 92278, 92277, 92276, 92275, 92274, 92273, 92272, 92271, 92270, 92287, 92288, 92289, 92332, 92331, 92324, 92323, 92322, 92321, 92320, 92319, 92318, 92317, 92316, 92305, 92304, 92303, 92291, 92290, 92270, 92268, 92267, 92246, 92245, 92243, 92242, 92241, 92240, 92239, 92238, 92237, 92236, 92235, 92234, 92233, 92232, 92231, 92230, 92247, 92248, 92250, 92265, 92264, 92263, 92262, 92261, 92260, 92259, 92258, 92257, 92256, 92255, 92254, 92253, 92252, 92251, 92250, - 92230View allHide

CF Dye Succinimidyl Ester

From: $147 Sizes: 0.25 umol, 1 umolCatalog #: 92137, 92106, 92107, 92108, 92109, 92110, 92111, 92112, 92117, 92123, 96011, 96014, 96016, 92105, 92104, 92139, 92142, 92150, 92127, 92130, 92131, 92132, 92133, 92134, 92135, 92155, 92103, - 92120View allHide

CF Dye Maleimide

From: $184 Sizes: 0.25 umol, 1 umolCatalog #: 92020, 92033, 92034, 92044, 92045, 92046, 92118, 92124, 92128, 96012, 96015, 92032, 92031, 92021, 92022, 92023, 92024, 92025, 92026, 92027, 92028, 92029, 92030, - 96017View allHide

CF® Dye Labeled & Biotinylated Secondary Antibodies

Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin.

Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.

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Faq

CF® Dyes

See our CF® Dye Quick Reference Table for a list of dyes and summary of their features. Our CF® Dye Selection Guide has more detailed information on each CF® dye, and ordering information for our various CF® dye product lines. You can download CF® dye normalized absorption and emission data in Excel format.

Category: Mix-n-Stain™ Antibody Labeling Kits, CF® Dyes

← FAQs
CF® initially was an abbreviation for Cyanine-based Fluorescent dyes. These were the first patented CF® dyes based on cyanine dye structures. Since then, our CF® dye patent portfolio has expanded to include four different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR.

Category: CF® Dyes

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The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.

Category: CF® Dyes

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