Extracellular Vesicle Research

ExoBrite™ Annexin EV Staining Kits are optimized for bright, sensitive EV detection across diverse sources. Like ExoBrite™ CTB stains, they offer high signal with little to no background aggregation, ideal for flow cytometry. They deliver broad EV coverage and strong performance across EVs from 9 tested cell lines—making them a powerful tool for consistent and reliable EV detection.

ExoBrite™ WGA EV Stains are fluorescent conjugates of wheat germ agglutinin (WGA) for bright and sensitive staining of EVs. Similar to ExoBrite™ Annexin EV Stains, these WGA conjugates offer broad compatibility with EVs. The stains were validated for EVs derived from all 9 cell lines tested. In addition, ExoBrite™ WGA EV Stains are less prone to aggregation than hydrophobic membrane dyes and may be used to stain purified and bead-bound EVs.

ExoBrite™ CTB EV Staining Kits are fluorescent conjugates of cholera toxin subunit B (CTB). They offer were extremely low background for detection of EVs by flow cytometry. Also, ExoBrite™ CTB EV stains do not bind non-specifically to polystyrene beads, meaning that they can be used to stain bead-bound EVs.

The ExoBrite™ EV Stain Enhancer is a unique additive that can be added to extracellular vesicle (EV) stain reactions to improve the staining specificity for applications like flow cytometry. The ExoBrite™ Stain Enhancer works by reducing the aggregation of certain EV stains, which allows the conjugate to stain the EVs more efficiently, resulting in a better signal-to-noise ratio and fewer false positives.

ExoBrite™ Flow Antibody Conjugates target key EV markers CD9, CD63, and CD81 with high sensitivity and low background. Carefully selected and validated for robust EV detection, they feature a proprietary buffer to reduce aggregation and enhance staining. Matching isotype controls are also available for accurate flow cytometry analysis of isolated EVs.

Biotium’s ExoBrite™ CD9/CD63/CD81 Antibody Cocktails offer optimal brightness and low background for EV detection by flow cytometry. Single-color cocktails enable high-coverage EV staining, while 3-color cocktails allow multiplexed detection and robust phenotyping with distinct fluorescent labels. Both formats are validated for low aggregation and high signal-to-noise.

Streamline your EV and exosome isolation workflows with ExoBrite™ Streptavidin Magnetic Beads, developed to offer lower background and higher sensitivity for EV capture than similar products. These streptavidin-coated and magnetic polystyrene beads (4.5 um diameter) may be used to isolate EVs from cell culture medium or other biological fluids without an overnight precipitation step.

ExoBrite™ Western Antibodies were validated to offer bright signal and low background of EV markers CD9, CD63, and CD81 in EV extracts by chemiluminescence or near-IR fluorescent western blot. The antibodies are offered conjugated to HRP or to the near-infrared fluorescent dyes ExoBrite™ 680/700 and ExoBrite™ 770/800, which provide higher signal-to-noise than dyes with visible light emission for western blotting.

An ExoBrite™ Calnexin Western Antibody detects a protein of the endoplasmic reticulum that is not found in EVs. It is offered as a negative control to assess the purity of isolated EV extracts.