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EvaGreen® Dye-Based qPCR Master Mixes
Features
- Competitive pricing: Just $20/mL (20 mL size)
- EvaGreen® Dye: Higher signal-to-noise, safer, and more eco-friendly over SYBR® Green
- Ideal for fast cycling: Cheetah™ Hotstart Taq activates in 2 min at 95°C
- 2-color tracking: Reduces pipetting errors, saving time, reagents, and samples
- Flexible ROX options: None, separate tube, or premixed (high/low concentration)
EvaGreen® Dye
EvaGreen® Dye is a unique, safe DNA-binding dye ideal for qPCR and HRM analysis*. Its novel "release-on-demand" mechanism allows high dye concentration without PCR inhibition (Mao et al., 2007, BMC Biotech). Unlike other dyes, EvaGreen® Dye cannot cross cell membranes, minimizing genotoxic risk. SYBR® Green I, by contrast, is more environmentally toxic than ethidium bromide (Ohta et al., 2001, Mut. Res.).
Independent studies confirm EvaGreen® is nonmutagenic, noncytotoxic, and safe for aquatic life, making it drain-disposable. For details, download the EvaGreen® Dye Safety Report.
As an added benefit, EvaGreen®-based master mixes enable direct gel analysis without adding extra stain; the dye serves as a built-in pre-stain for easy band visualization.
Cheetah™ HotStart Taq
Cheetah™ HotStart Taq is Biotium’s proprietary chemically modified Taq polymerase designed for reducing non-specific DNA amplification in PCR. Cheetah™ Taq features a novel chemical modification that completely suppresses its activity prior to activation, and offers a much faster activation time than AmpliTaq Gold® or HotStartTaq®. Cheetah™ HotStart Taq is available in two different formulations: Cheetah™ FLEX is formulated for fast-cycling qPCR and proximity extension assays (PEA), and Cheetah™ PEA-PLEX is formulated for NGS-based high-throughput PEA.
| Product | Reference Dye | Catalog No. | Size |
|---|---|---|---|
| Forget-Me-Not™ EvaGreen® qPCR Master Mix (Premixed Low ROX or High ROX) | Low ROX | 31045-T | 1 mL (100 reactions) |
| 31045-5mL | 5 x 1 mL (500 reactions) | ||
| 31045-20mL | 2 x 10 mL (2000 reactions) | ||
| High ROX | 31046-T | 1 mL (100 reactions) | |
| 31046-5mL | 5 x 1 mL (500 reactions) | ||
| 31046-20mL | 2 x 10 mL (2000 reactions) | ||
| Forget-Me-Not™ EvaGreen® qPCR Master Mix (2-Color Tracking) | No ROX | 31041-T | 1 mL (100 reactions) |
| 31041-1 | 5 x 1 mL (500 reactions) | ||
| 31041-20mL | 2 x 10 mL (2000 reactions) | ||
| Separate tube of ROX | 31042-T | 1 mL (100 reactions) | |
| 31042-1 | 5 x 1 mL (500 reactions) | ||
| 31042-20mL | 2 x 10 mL (2000 reactions) |
Universal Probe qPCR Master Mix
Forget-Me-Not™ Universal Probe qPCR Master Mix supports all fluorescent probe-based assays, including TaqMan®, BHQ®, and molecular beacons, with excellent results in singleplex and multiplex formats. Compatible with standard and fast protocols on most instruments. Includes optional blue Tracking Buffer to help identify wells with master mix or template. Available with or without ROX reference dye.
| Product | Reference Dye | Catalog No. | Size |
|---|---|---|---|
| Forget-Me-Not™ Universal Probe qPCR Master Mix | No ROX | 31043-T | 1 mL (100 reactions) |
| 31043-1 | 5 x 1 mL (500 reactions) | ||
| Separate tube of ROX | 31044-T | 1 mL (100 reactions) | |
| 31044-1 | 5 x 1 mL (500 reactions) |
Master Mix Selection Guide
| Catalog No. | Master Mix | EvaGreen® qPCR | Probe-Based PCR | Optimized for dPCR | 1-Color Tracking | 2-Color Tracking |
|---|---|---|---|---|---|---|
| 31041 | Forget-Me-Not™ EvaGreen® qPCR Master Mix with 2-Color Tracking | ✓ | ✓ | |||
| 31042 | Forget-Me-Not™ EvaGreen® qPCR Master Mix with 2-Color Tracking, with separate ROX | ✓ | ✓ | |||
| 31045 | Forget-Me-Not™ EvaGreen® qPCR Master Mix (low ROX) | ✓ | ✓ | |||
| 31046 | Forget-Me-Not™ EvaGreen® qPCR Master Mix (high ROX) | ✓ | ✓ | |||
| 31043 | Forget-Me-Not™ Universal Probe qPCR Master Mix (no ROX) | ✓ | ✓* | |||
| 31044 | Forget-Me-Not™ Universal Probe qPCR Master Mix, with separate ROX | ✓ | ✓* | |||
| 31078 | N-Flux™ 5X Digital PCR Master Mix | ✓ | ✓ |
EvaGreen® Dye and Cheetah™ HotStart Taq DNA Polymerase are covered under US and international patents. QuantiNova is a registered trademark of Qiagen Group. SYBR is a registered trademark of Molecular Probes, Inc./Life Technologies. *HRM is a registered trademark of Idaho Technologies, Inc./BioFire Defense, LLC, and its use may require a license.
