GloMelt™ Thermal Shift Protein Stability Kit

The GloMelt™  Thermal Shift Protein Stability Kit is used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

Also view our GloMelt™ 2.0 Thermal Shift Protein Stability Kit which features a Recombinant Monoclonal Mouse IgG1 for performing control reactions. Compared to the polyclonal goat IgG control provided in the original GloMelt™ kit, the recombinant mouse antibody included in the GloMelt™ 2.0 Kit generates higher signal and a sharper melt peak between for robust assay validation.

About GloMelt™ Thermal Shift Protein Stability Kit

Environmentally sensitive fluorescent dyes can be used to monitor the temperature dependent unfolding of a protein. The protein’s melting temperature (Tm) is a reporter of the protein’s thermal stability.

GloMelt™ dye undergoes fluorescence enhancement upon binding to hydrophobic regions of denatured proteins, and therefore can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

The thermal shift assay is a rapid and inexpensive technique that quantifies change in protein denaturation temperature, and thus can be used to screen conditions that affect protein thermal stability, such as protein mutations, ligand binding, and buffer formulations (like pH, salts, detergents, and other additives). These assays are rapid (typically about 30 minutes) and are performed on a quantitative PCR system. The thermal shift method is compatible with high throughput screening and requires much less protein than methods such as circular dichroism and differential scanning calorimetry.

To learn more about GloMelt™ and see how it compares to competitor products, visit our GloMelt™ Technology Page.

Possible Uses for Thermal Shift Assays

• Optimize buffer formulation for protein stability and storage
• Determine how mutations affect your protein’s stability
• Rapidly screen small molecule drug candidates and other ligands for protein binding

Small molecule inhibitors of bromodomain proteins have shown therapeutic effects in cancer models. Here, a GloMelt™ thermal shift assay was performed on 10 ug bromodomain BRD2 in the presence of bromodomain inhibitors JQ1 or RVX-208. Inhibitor binding stabilized the protein as indicated by the shift of the melting curves.