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September 1, 2022

Can CF® Dyes be used to label lipopolysaccharide (LPS) for in vivo studies?

By: Jacqueline Steenhuis

We do not have firsthand experience with LPS labeling, but according to the literature,  LPS has been labeled using amine-reactive dyes, like FITC. Our amine-reactive CF® Dye Succinimidyl Esters should also work for this. There is a publication for enzymatic labeling of LPS using dye hydrazides. Our CF® Dye hydrazides could be used in this method. The paper also describes the traditional amine labeling method and purification of the conjugate.