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How stable is CellBrite™ membrane staining? Are the dyes toxic to cells?

Lipophilic carbocyanine dyes like our original CellBrite™ Cytoplasmic Membrane Stains have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year (see note below). The dyes do not appreciably affect cell viability, and do not readily transfer between cells with intact membranes, allowing cell migration and tracking studies in mixed populations. Staining with the covalent stains CellBrite™ Fix and MemBrite™ Fix lasts up to 48 hours in tissue culture cells (see note below).

Note: Over time, all cell surface stains will be internalized and become intracellular as membranes turn over by endocytosis. The rate of internalization may vary by cell type, rate of membrane turnover, and rate of cell division. In immortalized cells in culture, most of the surface staining becomes internalized over the course of about 24 hours for CellBrite™, CellBrite™ Fix, and MemBrite™ Fix stains.

For an alternative stable and fixable stain for long term cell tracking or tracking cells in mixed cultures, see our ViaFluor® SE Cell Proliferation Dyes. These dyes covalently label intracellular proteins throughout the cell and are non-toxic and fixable.

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