Formalin-fixed tissue samples are a challenge for RNA extraction, often resulting in low yields and poor performance in subsequent steps. Most existing methods rely on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids. Powered by proprietary CAT5™ catalytic technology, the RNAstorm™ FFPE RNA Extraction Kits enhance the removal of formaldehyde-induced damage. The extracted RNA has higher yield, quality, and greater amplifiability that is more optimal for next-generation sequencing, qPCR, microarray, or other gene expression analysis.

The RNAstorm™ 2.0 MagBead FFPE RNA Extraction Kit uses magnetic beads for RNA isolation and may be adapted for multiwell plate format. For spin column-based RNA purification, see our original CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit.

• Purify higher quality RNA with less fragmentation
• Extract higher yields of amplifiable RNA compared to other methods
• CAT5™ technology for chemical reversal of formaldehyde crosslinking
• Milder conditions and no harsh solvents
• Better results in downstream analysis like PCR, microarray, or next-generation sequencing
• Magnetic bead-based RNA purification adaptable for multiwell plate formats

High-Throughput and Efficient RNA Extraction from FFPE Tissues

The RNAstorm™ 2.0 MagBead FFPE RNA Extraction Kit offers the same performance and CAT5™ technology for efficient removal of formaldehyde crosslinking found in our spin column-based CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit.

Comparison of RNA recovered from human kidney FFPE sections using the CELLDATA RNAstorm™ 2.0 FFPE RNA Extraction Kit using MagBeads or spin columns. RNA was isolated from four 10 um FFPE sections per sample using duplicate samples for each kit. Eluted RNA (15 uL per lane) was analyzed on a 1.5% EMBER™ Ultra RNA agarose gel and imaged on Biotium’s Gel-Bright® Laser Diode Gel Illuminator. The RNAstorm™ 2.0 FFPE MagBead FFPE RNA Kit yielded RNA with comparable recovery and RNA size profiles as the RNAstorm™ 2.0 kit with spin columns. Ladder: NEB Low Range RNA Ladder. Lanes 1, 2: RNAstorm™ FFPE spin column eluates. Lanes 3, 4: RNAstorm™ FFPE MagBead eluates.

Advantages of CAT5™ Technology for RNA Extraction

Higher Yields of Amplifiable RNA

Significant improvements in RNA yield and quality (as measured by amount of amplifiable RNA) are seen using a prototype of the RNAstorm™ kit featuring CAT5™ technology on FFPE samples from various tissues and with collection dates ranging from 1976 to 2015 compared to a competitor kit.

Comparison of RNA recovery by quantitative RT-PCR from FFPE tissues. “Q” represents a competitive commercial FFPE extraction kit.

Higher Integrity RNA

Increased DV200 values are observed for RNA extracted using the RNAstorm™ kit relative to a popular commercial kit. The DV200 represents percentage of RNA with length greater than 200 nt, as measured using an Agilent Bioanalyzer RNA 6000 nano kit.

An exemplary overlay of BioAnalyzer traces obtained using RNA from the same sample as seen with the RNAstorm™ kit and Kit Q is shown below.

See the product table below for our complete line of RNAstorm™ and DNAstorm™ extraction kits for FFPE tissues. We also offer the RNAstorm™ RNA Isolation Kit for purifying RNA from fresh or frozen cells/tissue samples, and the ExoBrite™ EV Total RNA Isolation Kit for isolate RNA from extracellular vesicles.

RNAstorm™ and DNAstorm™ FFPE Extraction Kits