CF® dye with BCN, an alternative to fluorescently labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.
CF dyes are exceptionally bright and photostable, making it the perfect dye for fluorescence detection. The following CF dyes are available with BCN: CF®405S, CF®405M, CF®488A, CF®500, CF®568, CF®594, CF®640R, CF®647, CF®650, CF®680, and CF®680R.
CF®500 BCN and CF®650 BCN are designed to use for intracellular copper-free reaction with azide.
CF Dye BCN: Product & SKU Summary