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CF® Dye BCN

CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.

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Product Description

CF® dye with BCN, an alternative to fluorescently labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper.

CF dyes are exceptionally bright and photostable, making it the perfect dye for fluorescence detection. The following CF dyes are available with BCN: CF®405S, CF®405M, CF®488A, CF®500, CF®568, CF®594, CF®640R, CF®647, CF®650, CF®680, and CF®680R.

CF®500 BCN and CF®650 BCN are designed to use for intracellular copper-free reaction with azide.

CF Dye BCN: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase
CF®405M BCNCF®405M408/452 nm0.5 mg92114Purchase 92114
CF®405S BCNCF®405S404/431 nm0.5 mg92113Purchase 92113
CF®488A BCNCF®488A490/515 nm0.5 mg92075Purchase 92075
CF®500 BCNCF®500500/510 nm0.5 mg96026Purchase 96026
CF®568 BCNCF®568562/583 nm0.5 mg92076Purchase 92076
CF®594 BCNCF®594593/614 nm0.5 mg92077Purchase 92077
CF®640R BCNCF®640R642/662 nm0.5 mg92078Purchase 92078
CF®650 BCNCF®650650/670 nm0.5 mg96027Purchase 96027
CF®680R BCNCF®680R680/701 nm0.5 mg92079Purchase 92079

References

Download a list of CF® dye references.

 

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    CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.

    CF® Dye Azide

    CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

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    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.