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CF® Dye BCN

CF® dye with BCN reacts with azide to form 1,2,3-triazole by copper-free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction allows staining the surface of live cells or cell extracts when there are concerns about native protein function loss with copper. We also offer membrane-permeant CF® dye BCN options for intracellular reaction.

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Product Description

CF® dye with BCN, an alternative to fluorescently labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper-free 1,3-dipolar Huisgen cycloaddition. This copper-free bioorthogonal reaction can be used with live cells or cell extracts when there are concerns about native protein function loss with copper.

CF® dyes are exceptionally bright and photostable, making them the perfect dyes for fluorescence detection. The following CF® dyes are available with BCN: CF®405S, CF®405M, CF®440, CF®488A, CF®500, CF®568, CF®594, CF®640R, CF®647, CF®650, CF®680, and CF®680R.

CF®440 BCN, CF®500 BCN, and CF®650 BCN are membrane-permeant for intracellular copper-free reaction with azide.

ProductEx/EmMembrane PermeabilitySizeCatalog number
CF®405S BCN404/431 nmImpermeant0.5 mgPurchase 92113
CF®405M BCN408/452 nmImpermeant0.5 mgPurchase 92114
CF®440 BCN440/515 nmPermeant0.5 mgPurchase 96070
CF®488A BCN490/515 nmImpermeant0.5 mgPurchase 92075
CF®500 BCN500/510 nmPermeant0.5 mgPurchase 96026
CF®568 BCN562/583 nmImpermeant0.5 mgPurchase 92076
CF®594 BCN593/614 nmImpermeant0.5 mgPurchase 92077
CF®640R BCN642/662 nmImpermeant0.5 mgPurchase 92078
CF®647 BCN650/665 nmImpermeant0.5 mgPurchase 96059
CF®650 BCN650/670 nmPermeant0.5 mgPurchase 96027
CF®680 BCN681/698 nmImpermeant0.5 mgPurchase 96058
CF®680R BCN680/701 nmImpermeant0.5 mgPurchase 92079

References

Download a list of CF® dye references.

 

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    CF® dye alkyne reacts with azide to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It can also be used as a monomer to copolymerize with other monomers to form fluorescent polymers.

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    CF® dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalyzed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO,  reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition. This copper free bioorthogonal reaction allows staining the surface of live cells or having concerns about native protein function loss with copper in cell extracts.

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    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.