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CF® dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® dye BCN conjugates for intracellular labeling reactions are also available.

Product Attributes

Functional group


Chemical reactivity (reacts with)

Azides/Picolyl azides

Storage Conditions

Store at -10 to -35 °C, Protect from light

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

CF® dyes BCN (bicyclo[6.1.0]nonyne) derivatives are alternatives to fluorescently labeled DIBO and DBCO. They react with azides to form 1,2,3-triazole by copper-free bioorthogonal 1,3-dipolar Huisgen cycloaddition. Useful for fluorescent surface labeling of live cells or cell extracts, particularly when there are concerns about native protein function loss with copper. Membrane-permeant CF® dye BCN options are also available for intracellular labeling reactions.

Available CF® dye BCN derivatives: CF®405S, CF®405M, CF®488A, CF®568, CF®594, CF®640R, CF®647, CF®680, and CF®680R including membrane-permeant CF®440 BCN, CF®500 BCN, and CF®650 BCN.

  • Fluorescent labeling of azide groups on target molecules via copper-free bioorthonal chemistry.
  • Alternative to fluorescently labeled DIBO and DBCO.
  • Suitable for surface or intracellular labeling of live cells or cell extracts.
  • Bright, photostable and water-soluble CF® dyes are excellent options for fluorescent labeling.
  • Available in several CF® dye options including membrane-permeant forms for intracellular labeling.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

ProductEx/EmMembrane PermeabilitySizeCatalog number
CF®405S BCN404/431 nmImpermeant0.5 mgPurchase 92113
CF®405M BCN408/452 nmImpermeant0.5 mgPurchase 92114
CF®440 BCN440/515 nmPermeant0.5 mgPurchase 96070
CF®488A BCN490/515 nmImpermeant0.5 mgPurchase 92075
CF®500 BCN500/510 nmPermeant0.5 mgPurchase 96026
CF®568 BCN562/583 nmImpermeant0.5 mgPurchase 92076
CF®594 BCN593/614 nmImpermeant0.5 mgPurchase 92077
CF®640R BCN642/662 nmImpermeant0.5 mgPurchase 92078
CF®647 BCN650/665 nmImpermeant0.5 mgPurchase 96059
CF®650 BCN650/670 nmPermeant0.5 mgPurchase 96027
CF®680 BCN681/698 nmImpermeant0.5 mgPurchase 96058
CF®680R BCN680/701 nmImpermeant0.5 mgPurchase 92079


1. Canadian Journal of Chemistry (2019),97(1):1-6. DOI: 10.1139/cjc-2018-0253.
2. eLife (2019),8: e50776. DOI: 10.7554/eLife.50776.001
3. Chem. Mater. (2019), 31:8035−8043. DOI: 10.1021/acs.chemmater.9b02485
4. Advanced functional materials, (2016),26(21):3612-3620. DOI: 10.1002/adfm.201505329

Download a list of CF® dye references.


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    CF® Dye Alkyne

    CF® dye alkynes react with azide groups via copper-catalyzed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® dye alkynes can also be used as building blocks to form fluorescent polymers.

    CF® Dye Azide

    CF® dyes with an azide group. Can be used to label or detect alkyne, BCN or cyclooctyne-containing molecules by fluorescence spectroscopy, microscopy or flow cytometry following copper-catalyzed azide-alkyne cycloaddition.

    Biotin BCN

    Biotin BCN, an alternative to biotin labeled DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.

    CF® Dye Succinimidyl Ester

    CF® dye SE or NHS esters are amine-reactive fluorescent dyes. They are commonly used to label primary amines of antibodies and other proteins, amine-modified oligonucleotides and other amine-containing molecules.

    DMSO, Anhydrous

    Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions of AM ester dyes, reactive dyes and other related moisture-sensitive products.

    CF® Dye Picolyl Azide

    CF® dye picolyl azide derivatives fluorescently label alkyne groups on biomolecules via the standard azide-alkyne cycloaddition, but at low copper concentrations.