Di-2-ANEPEQ, also called JPW1114, is a fast-responding membrane potential dye. It is highly water-soluble and is usually introduced into cells by microinjection.
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Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.
DiBAC4(3) (Bis-(1,3-Dibarbituric acid)-trimethine oxanol) is a so-called translational membrane potential dye that redistributes within the cell membrane when membrane potential changes (1,2). The fluorescence of the dye is enhanced when the dye enters the cell membrane as a result of membrane depolarization.
ANEPPS dyes are fast responding membrane potential dyes. Compared to Di-4-ANEPPS, Di-8-ANEPPS is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS
Di-8-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-2-ANEPEQ and is useful for potential-sensitive retrograde labeling of neurons.
Di-12-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-8-ANEPPQ and is useful for potential-sensitive retrograde labeling of neurons.