Di-4-ANEPPS
Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye.
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Product Description
Di-4-ANEPPS is a fast-responding membrane potential dye (1). Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye. Di-4-ANEPPS is internalized in cells rapidly, so it is mainly used for short-term experiments. We also offer Di-8-ANEPPS (catalog number 61012), which is more lipophilic and better retained in the outer leaflet of the plasma membrane.
- λEx/λEm (MeOH) = 496/705 nm*
- Orange red solid soluble in DMF, ethanol or DMSO
- Store at 4°C and protect from light, especially in solution
- C28H36N2O3S
- MW: 481
- [90134-00-2]
* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.
References
- Nature, 355, 349(1992), DOI: 10.1038/355349a0
- Biochim Biophys Acta, 1068(2), 149(1991), DOI: 10.1016/0005-2736(91)90203-K
- PLoS ONE 8(10), e75678 (2013), DOI: 10.1371/journal.pone.0075678
- Am J Phys Heart, 0(0), (2021), DOI: 10.1152/ajpheart.00185.2021