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Di-8-ANEPPS

ANEPPS dyes are fast responding membrane potential dyes. Compared to Di-4-ANEPPS, Di-8-ANEPPS is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS

Product Attributes

CAS number

157134-53-7

For live or fixed cells

For live/intact cells

Potential dependence

Fast-response membrane potential dye

Colors

Far-red

Excitation/Emission

498/713 (see product description)

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5 mg
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Product Description

ANEPPS dyes are fast responding membrane potential dyes (1). Biotium offers both Di-4-ANEPPS and Di-8-ANEPPS. Di-4-ANEPPS has the problem of being rapidly internalized in cells, so it is mainly used for short-term experiments. However, because Di-8-ANEPPS is more lipophilic, the dye is better retained in the outer leaflet of cell plasma membranes and thus more suitable for long-term membrane potential studies. Di-8-ANEPPS is also more photostable and less phototoxic than Di-4-ANEPPS (2,3).

  • λEx/λEm (MeOH) = 498/713 nm*
  • Orange red solid soluble in DMF, ethanol or DMSO
  • Store at 4°C and Protect from light, especially when in solution
  • C36H52N2O3S
  • MW:593
  • [157134-53-7]

* Note that the spectra of styryl dyes generally undergo a large blue shift in lipids compared to organic solvent. See the spectra for styryl dyes in liposomes under product images.

References

1. J Neurosci 15, 1392 (1995).

2. Neuron 13, 1187 (1994).

3. Neuron 9, 393 (1992).

4. International Society for Advancement of Cytometry (2015), doi: 10.1002/cyto.a.22787

 

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