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DiI

DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C18 carbon) hydrocarbon chains into the lipid bilayer. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons.

Product Attributes

CAS number

41085-99-8

Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Red

Excitation/Emission

549/565 nm

Size
Catalog #
price
Qty
50 mg
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Product Description

DiI is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C18 carbon) hydrocarbon chains into the lipid bilayer. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons. The intense fluorescence and high photostability of the dye make it possible to visualize the fine structures (axons and dendrites) of the neurons. Due to its low toxicity and the tendency to give highly stable cell labeling, the dye has also been generally used for long term cell tracing of cells both in cultures and in living embryos or animals. Please also see CellBrite™ Orange, a DiI solution for labeling cell membranes, and DiI in Vegetable Oil, a ready-to-use solution for microinjection studies.

  • DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
  • λExEm (MeOH) = 549/565 nm
  • ε = 148,000
  • Dark red solid soluble at 1-2 mM (with heating) in DMF, DMSO, or ethanol
  • Soluble at 1-2 mM in vegetable oil with heating and sonication
  • Store at 4°C
  • C59H97ClN2O4
  • MW: 933.88
  • [41085-99-8]

 

References

1. Biochemistry 19, 6050 (1980)
2. J. Immunology 127, 893 (1981)
3. Trends in Neurosci 9, 333 (1989)
4. J Cell Biol 103, 171 (1986)

 

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