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Neuro-DiI

Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI. Neuro-DiI has structural features that may make the probe diffuse faster than DiI on cell membranes and also may result in a more stable labeling.

Product Attributes

Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Red

Excitation/Emission

549/565 nm

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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25 mg
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Product Description

Neuro-DiI was developed at Biotium as an alternative to the widely used fluorescent membrane probe DiI. Like Dilinoleyl DiI, Neuro-DiI has structural features that may make the probe diffuse faster than DiI on cell membranes. However, Neuro-DiI dye has saturated carbon chains, making it more hydrophobic than Dilinoleyl DiI, for potentially more stable labeling with less dye transfer between cells. Neuro-DiI has nearly identical absorption and emission wavelengths to those of DiI. We also offer Neuro-DiI in Vegetable Oil for microinjection studies. Also see our CellBrite™ Orange Cytoplasmic Membrane Dye, a ready-to-use dye solution for cell labeling.

  • λExEm (MeOH) = 549/565 nm
  • ε = 148,000
  • Red solid soluble at 1-2 mM (with heating) in DMF, DMSO, or ethanol
  • Soluble at 1-2 mM in vegetable oil with heating and sonication
  • Store at 4°C and protect from light
  • C67H115IN2
  • MW: 1072

References

1. Proc Natl Acad Sci (2006) Feb 21;103(8):2938-42 doi: 10.1073/pnas.0511159103
2. J Neurosci. (2013) Oct 23;33(43):16853-64 doi: 10.1523/JNEUROSCI.1844-13.2013
3. PLoS One (2013) May 31;8(5):e64907 doi: 10.1371/journal.pone.0064907
4. J Neurosci (2013) Apr 10;33(15):6454-9 doi: 10.1523/JNEUROSCI.0178-13.2013
5. Bioconjug Chem (2014) Dec 17;25(12):2134-43 doi: 10.1021/bc500465j
6. Front Syst Neurosci (2016) Nov 15;10:95 doi: 10.3389/fnsys.2016.00095

 

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