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DiI in Vegetable Oil

DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) uniformly dissolved in vegetable oil in a formulation optimized for microinjection. Carbocyanine microinjection has been widely used for fate mapping and cell lineage studies and for the determination of spatial organization and connectivity patterns of central nervous systems.

Product Attributes

CAS number

41085-99-8

Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Red

Excitation/Emission

549/565 nm

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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0.5 mL
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Product Description

DiI uniformly dissolved in vegetable oil in a formulation optimized for microinjection. Carbocyanine microinjection has been widely used for fate mapping and cell lineage studies and for the determination of central nervous system spatial organization and connectivity patterns. The red fluorescent DiI and green fluorescent Neuro-DiO in oil can be used in combination for two color imaging. We also offer DiI solid, and CellBrite™ Orange, a DiI solution for staining cell membranes.

  • DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
  • λExEm (MeOH) = 549/565 nm
  • Red oil
  • Store at room temperature and protect from light (do not freeze)
  • [41085-99-8]

 

References

1. Ruiz, et al. "Fate maps and cell lineage analysis" in Essential developmental biology: a practical approach. Oxford: IRL Press at Oxford University Press, 81-95 (1993).

2. Int J Parasitol 28, 363 (1998).

 

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