DiI in Vegetable Oil
DiI (DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) uniformly dissolved in vegetable oil in a formulation optimized for membrane labeling or cell tracking. Carbocyanine dyes in oil have been widely used for fate mapping, cell lineage studies, pre-labeling cells for transplantation, and neuronal tracing and labeling.
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Product Description
DiI uniformly dissolved in vegetable oil in a formulation optimized for membrane labeling or cell tracking. Carbocyanine dyes in oil have been widely used for fate mapping and cell lineage studies. 1-2 These dyes are used for pre-labeling neurons for transplantation, tracing cranial or spinal nerves in fixed brain tissue, and labeling of nerves in tracing studies as well. 3-5 The red fluorescent DiI and green fluorescent Neuro-DiO in oil can be used in combination for two color imaging. We also offer DiI solid, and CellBrite® Orange, a DiI solution for staining cell membranes.
- DiIC18(3); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
- λEx/λEm (MeOH) = 549/565 nm
- Red oil
- Store at room temperature and protect from light (do not freeze)
- [41085-99-8]
References
- Ruiz, et al. “Fate maps and cell lineage analysis” in Essential developmental biology: a practical approach. Oxford: IRL Press at Oxford University Press, 81-95 (1993).
- McPherson, et al. “Cortical Localization of a Calcium Release Channel in Sea Urchin Eggs.” Journal of Cell Biology 116, no. 5 (March 1992): 1111–1121. DOI:10.1083/jcb.116.5.1111.
- Potter, et al. “Neural Transplant Staining with DiI and Vital Imaging by 2-Photon Laser-Scanning Microscopy.” Scanning Microscopy Supplement 10 (1996): 189–199. PMID: 9601539.
- von Bartheld, et al. “Neuronal Tracing with DiI: Decalcification, Cryosectioning, and Photoconversion for Light and Electron Microscopic Analysis.” Journal of Histochemistry & Cytochemistry 38, no. 5 (May 1990): 725–733. DOI:10.1177/38.5.2185313.
- Murphy, et al. “Anterograde Tracing Method Using DiI to Label Vagal Innervation of the Embryonic and Early Postnatal Mouse Gastrointestinal Tract.” Journal of Neuroscience Methods 163, no. 2 (July 30, 2007): 213–225. DOI:10.1016/j.jneumeth.2007.03.001.